Differential expression and immunoreactivity of thyroid hormone transporters MCT8 and OATP1C1 in rat ovary

被引：2

作者：

Luis, Enoch ^{[1
,2
]}

Fernandez, Yesenia ^{[3
]}

Alvarado, Mayvi ^{[4
]}

Juarez-Santacruz, Libertad ^{[5
]}

Garcia-Nieto, Edelmira ^{[5
]}

Anaya-Hernandez, Arely ^{[3
,5
]}

机构：

[1] Univ Autonoma Mexico, Inst Fisiol Celular, Catedra CONACYT Lab Nacl Canalopatias, Cdmx, Mexico

[2] Univ Nacl Autonoma Mexico, Lab Nacl Canalopatias, Cdmx, Mexico

[3] Univ Autonoma Tlaxcala, Fac Ciencias Salud, PE Quim Clin, Tlaxcala, Mexico

[4] Univ Veracruzana, Inst Neuroetol, Dept Neurofisiol, Xalapa, Veracruz, Mexico

[5] Univ Autonoma Tlaxcala, Ctr Invest Genet & Ambiente, Autopista Tlaxcala San Martin,Km 10-5, Ixtacuixtla 90120, Tlaxcala, Mexico

来源：

ACTA HISTOCHEMICA | 2019年 / 121卷 / 08期

关键词：

Monocarboxylate transporter; Organic anion transporting polypeptide; Thyroid hormone transporter; Ovary; Female reproduction; FOLLICLE-STIMULATING-HORMONE; FUNCTIONAL-CHARACTERIZATION; TISSUE DISTRIBUTION; CORPUS-LUTEUM; IDENTIFICATION; GRANULOSA; PROLIFERATION; ANGIOGENESIS; MUTATIONS; RECEPTORS;

D O I：

10.1016/j.acthis.2019.151440

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Thyroid hormones (THs) regulate several physiological processes in female mammals, many of which are related to reproduction such as steroidogenesis in the ovary, oocyte and granulosa cells maturation, follicular development and differentiation, and ovulation. THs actions require the presence of THs transporters to facilitate their cellular uptake and efflux. MCT8 and OATP1C1 are the principal THs transporters. The aim of the present study was to determine the gene expression and cellular localization of MCT8 and OATP1C1 in the rat ovary during the diestrus-II cycle phase. Ovaries of virgin adult rats were histologically processed. Reverse Transcription-PCR and immunohistochemistry analyses for MCT8 and OATP1C1 were done. MCT8 gene expression level was significantly higher (P < 0.01) than that of OATP1C1 in the rat ovary. MCT8 and OATP1C1 were found in all types of ovarian cells but with different immunoreactivity. MCT8 showed stronger immunoreactivity in tertiary and Graafian follicles, corpus luteum and blood vessels, whereas OATP1C1's immunoreactivity was stronger in stroma cells, tunica albuginea, and blood vessels. Our results provide evidence that THs and their transporters are both necessary for ovarian function and that any alteration in these transporters could interfere with reproductive processes such as ovulation and steroidogenesis, compromising fertility.

引用

页数：8

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