In vitro organogenesis from internodal segments of adult sweet orange plants

被引：10

作者：

Bassan, Meire Menezes ^{[1
]}

Alves Mourao Filho, Francisco de Assis ^{[1
,2
]}

Miyata, Luzia Yuriko ^{[1
]}

Januzzi Mendes, Beatriz Madalena

机构：

[1] USP, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP, Brazil

[2] USP, Ctr Energia Nucl Agr, BR-13409970 Piracicaba, SP, Brazil

来源：

PESQUISA AGROPECUARIA BRASILEIRA | 2011年 / 46卷 / 06期

关键词：

Citrus sinensis; adventitious buds; biotechnology; plant growth regulators; GENETIC-TRANSFORMATION; CITRUS-SINENSIS; MATURE TISSUES; REGENERATION;

D O I：

10.1590/S0100-204X2011000600015

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pera', and 'Valencia' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27 degrees C in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regeneration was assessed 50 and 70 days after in vitro culture. Organogenesis in cultivars Hamlin and Valencia was promoted by EME supplemented with BAP, while NAA showed no apparent effect.

引用

页码：672 / 674

页数：3

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