Analysis of pork adulteration in commercial meatballs targeting porcine-specific mitochondrial cytochrome b gene by TaqMan probe real-time polymerase chain reaction

被引：102

作者：

Ali, M. E. ^{[1
]}

Hashim, U. ^{[2
]}

Mustafa, S. ^{[3
]}

Man, Y. B. Che ^{[3
]}

Dhahi, Th S. ^{[2
]}

Kashif, M. ^{[2
]}

Uddin, Md Kamal ^{[4
]}

Abd Hamid, S. B. ^{[1
]}

机构：

[1] Univ Malaya, Nanotechnol & Catalysis Res Ctr, Kuala Lumpur 50603, Malaysia

[2] Univ Malaysia Perlis, Inst Nano Elect Engn, Kangar 01000, Perlis, Malaysia

[3] Univ Putra Malaysia, Halal Prod Res Inst, Upm Serdang 43400, Selangor, Malaysia

[4] Univ Putra Malaysia, Inst Trop Agr, Upm Serdang 43400, Selangor, Malaysia

来源：

MEAT SCIENCE | 2012年 / 91卷 / 04期

关键词：

Meatball formulation; Double quenched TaqMan probe; Halal and Kosher foods; Threshold cycle; Ground meat; PCR; QUANTIFICATION; IDENTIFICATION; LENGTH; RAW;

D O I：

10.1016/j.meatsci.2012.02.031

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A test for assessing pork adulteration in meatballs, using TaqMan probe real-time polymerase chain reaction, was developed. The assay combined porcine-specific primers and TaqMan probe for the detection of a 109 bp fragment of porcine cytochrome b gene. Specificity test with 10 ng DNA of eleven different species yielded a threshold cycle (Ct) of 15.5 +/- 0.20 for the pork and negative results for the others. Analysis of beef meatballs with spiked pork showed the assay can determine 100-0.01% contaminated pork with 102% PCR efficiency, high linear regression (r(2)=0.994) and <= 6% relative errors. Residuals analysis revealed a high precision in all determinations. Random analysis of commercial meatballs from pork, beef, chicken, mutton and goat, yielded a Ct between 15.89 +/- 0.16 and 16.37 +/- 0.22 from pork meatballs and negative results from the others, showing the suitability of the assay to determine pork in commercial meatballs with a high accuracy and precision. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：454 / 459

页数：6

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