RAB7 is a small GTPase that belongs to the Rab family, and as a vesicle trafficking factor it is shown to regulate the transport to late endocytic compartments, autophagosome maturation and organelle function. In present study, we showed the critical roles of RAB7 GTPase on actin dynamics and mitochondria function in oocyte meiosis. RAB7 mainly accumulated at cortex and spindle periphery during oocyte maturation. RAB7 depletion caused the failure of polar body extrusion and asymmetric division, and Rab7 exogenous mRNA supplement could rescue the defects caused by RAB7 RNAi. Based on mass spectrometry analysis, we found that RAB7 associated with several actin nucleation factors and mitochondria-related proteins in oocytes. The depletion of RAB7 caused the decrease of actin dynamics, which further affected meiotic spindle migration to the oocyte cortex. In addition, we found that RAB7 could maintain mitochondrial membrane potential and the mitochondrial distribution in mouse oocytes, and this might be due to its effects on the phosphorylation of DRP1 at Ser616 domain. Taken together, our data indicated that RAB7 transported actin nucleation factor for actin polarization, which further affected the phosphorylation of DRP1 for mitochondria dynamics and the meiotic spindle migration in mouse oocytes.
机构:
CNRS, UMR7241, INSERM, CIRB,Coll France,U1050, F-75231 Paris 05, France
Equipe Labellise Ligue Canc, F-75013 Paris, France
Memolife Lab Excellence & Paris Sci Lettre, F-75005 Paris, FranceCNRS, UMR7241, INSERM, CIRB,Coll France,U1050, F-75231 Paris 05, France
Almonacid, Maria
Terret, Marie-Emilie
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机构:
CNRS, UMR7241, INSERM, CIRB,Coll France,U1050, F-75231 Paris 05, France
Equipe Labellise Ligue Canc, F-75013 Paris, France
Memolife Lab Excellence & Paris Sci Lettre, F-75005 Paris, FranceCNRS, UMR7241, INSERM, CIRB,Coll France,U1050, F-75231 Paris 05, France
Terret, Marie-Emilie
Verlhac, Marie-Helene
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机构:
CNRS, UMR7241, INSERM, CIRB,Coll France,U1050, F-75231 Paris 05, France
Equipe Labellise Ligue Canc, F-75013 Paris, France
Memolife Lab Excellence & Paris Sci Lettre, F-75005 Paris, FranceCNRS, UMR7241, INSERM, CIRB,Coll France,U1050, F-75231 Paris 05, France
机构:
UCL, Dept Cell & Dev Biol, Div Biosci, London WC1E 6BT, EnglandUCL, Dept Cell & Dev Biol, Div Biosci, London WC1E 6BT, England
Dalton, Caroline M.
Carroll, John
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UCL, Dept Cell & Dev Biol, Div Biosci, London WC1E 6BT, England
Monash Univ, Sch Biomed Sci, Dept Anat & Dev Biol, Melbourne, Vic 3800, AustraliaUCL, Dept Cell & Dev Biol, Div Biosci, London WC1E 6BT, England
机构:
MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA
Harvard Univ, Sch Med, Dept Surg, Massachusetts Gen Hosp, Boston, MA 02115 USAMIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA
Desai, Salil P.
Bhatia, Sangeeta N.
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MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA
Howard Hughes Med Inst, Chevy Chase, MD USAMIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA
Bhatia, Sangeeta N.
Toner, Mehmet
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机构:
MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA
Harvard Univ, Sch Med, Dept Surg, Massachusetts Gen Hosp, Boston, MA 02115 USAMIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA
机构:
CNRS, UMR7241, INSERM, CIRB,Coll France,U1050, F-75231 Paris 05, France
Equipe Labellise Ligue Canc, F-75013 Paris, France
Memolife Lab Excellence & Paris Sci Lettre, F-75005 Paris, FranceCNRS, UMR7241, INSERM, CIRB,Coll France,U1050, F-75231 Paris 05, France
Almonacid, Maria
Terret, Marie-Emilie
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h-index: 0
机构:
CNRS, UMR7241, INSERM, CIRB,Coll France,U1050, F-75231 Paris 05, France
Equipe Labellise Ligue Canc, F-75013 Paris, France
Memolife Lab Excellence & Paris Sci Lettre, F-75005 Paris, FranceCNRS, UMR7241, INSERM, CIRB,Coll France,U1050, F-75231 Paris 05, France
Terret, Marie-Emilie
Verlhac, Marie-Helene
论文数: 0引用数: 0
h-index: 0
机构:
CNRS, UMR7241, INSERM, CIRB,Coll France,U1050, F-75231 Paris 05, France
Equipe Labellise Ligue Canc, F-75013 Paris, France
Memolife Lab Excellence & Paris Sci Lettre, F-75005 Paris, FranceCNRS, UMR7241, INSERM, CIRB,Coll France,U1050, F-75231 Paris 05, France
机构:
UCL, Dept Cell & Dev Biol, Div Biosci, London WC1E 6BT, EnglandUCL, Dept Cell & Dev Biol, Div Biosci, London WC1E 6BT, England
Dalton, Caroline M.
Carroll, John
论文数: 0引用数: 0
h-index: 0
机构:
UCL, Dept Cell & Dev Biol, Div Biosci, London WC1E 6BT, England
Monash Univ, Sch Biomed Sci, Dept Anat & Dev Biol, Melbourne, Vic 3800, AustraliaUCL, Dept Cell & Dev Biol, Div Biosci, London WC1E 6BT, England
机构:
MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA
Harvard Univ, Sch Med, Dept Surg, Massachusetts Gen Hosp, Boston, MA 02115 USAMIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA
Desai, Salil P.
Bhatia, Sangeeta N.
论文数: 0引用数: 0
h-index: 0
机构:
MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA
Howard Hughes Med Inst, Chevy Chase, MD USAMIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA
Bhatia, Sangeeta N.
Toner, Mehmet
论文数: 0引用数: 0
h-index: 0
机构:
MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA
Harvard Univ, Sch Med, Dept Surg, Massachusetts Gen Hosp, Boston, MA 02115 USAMIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA