Carboxyl-coated magnetic nanoparticles for mRNA isolation and extraction of supercoiled plasmid DNA

被引：55

作者：

Sarkar, Tapasree Roy ^{[1
,2
]}

Irudayaraj, Joseph ^{[1
,2
,3
]}

机构：

[1] Purdue Univ, Purdue Canc Ctr, W Lafayette, IN 47907 USA

[2] Purdue Univ, Bindley Biosci Ctr, W Lafayette, IN 47907 USA

[3] Purdue Univ, Birck Nanotechnol Ctr, W Lafayette, IN 47907 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2008年 / 379卷 / 01期

关键词：

D O I：

10.1016/j.ab.2008.04.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Carboxyl-coated magnetic nanoparticles (MNPs) were used to demonstrate dual functionality: isolation of messenger RNA (mRNA) from mammalian cells and extraction of the supercoiled (sc) form of plasmid DNA (pDNA) from agarose gel. These MNPs were attached with 5'-NH2-tagged oligo-(dT)(25) primer and were used to isolate mRNA from breast cancer cells. The isolated mRNA was used for amplification of beta-actin to confirm the compatibility. These MNPs were also used to extract the sc form of pDNA from agarose gel. The compatibility of the pDNA was demonstrated by restriction digestion. Both of these methodologies are simple, inexpensive (compared with existing kits), and efficient. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：130 / 132

页数：3

共 18 条

[1] Application of superparamagnetic nanoparticles in purification of plasmid DNA from bacterial cells [J].

Chiang, CL ;

Sung, CS ;

Wu, TF ;

Chen, CY ;

Hsu, CY .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2005, 822 (1-2) :54-60

[2] Improved manufacture and application of an agarose magnetizable solid-phase support [J].

Davies, MJ ;

Smethurst, DE ;

Howard, KM ;

Todd, M ;

Higgins, LM ;

Bruce, IJ .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1997, 68 (1-2) :95-112

[3] Identification and characterization of the hypoxia-responsive element of the human placental 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene [J].

Fukasawa, M ;

Tsuchiya, T ;

Takayama, E ;

Shinomiya, N ;

Uyeda, K ;

Sakakibara, R ;

Seki, S .

JOURNAL OF BIOCHEMISTRY, 2004, 136 (03) :273-277

[4] DNA PURIFICATION AND ISOLATION USING A SOLID-PHASE [J].

HAWKINS, TL ;

OCONNORMORIN, T ;

ROY, A ;

SANTILLAN, C .

NUCLEIC ACIDS RESEARCH, 1994, 22 (21) :4543-4544

[5]

Hegen P N, 1994, Trends Biochem Sci, V19, P388

[6] Magnetic and gold-coated magnetic nanoparticles as a DNA sensor [J].

Kouassi, Gilles K. ;

Irudayaraj, Joseph .

ANALYTICAL CHEMISTRY, 2006, 78 (10) :3234-3241

[7]

LI QZ, 1993, BIOTECHNIQUES, V15, P976

[8] Nitrilotriacetic acid-coated magnetic nanoparticles as affinity probes for enrichment of histidine-tagged proteins and phosphorylated peptides [J].

Li, Yi-Cheng ;

Lin, Ya-Shiuan ;

Tsai, Pei-Jane ;

Chen, Cheng-Tai ;

Chen, Wei-Yu ;

Chen, Yu-Chie .

ANALYTICAL CHEMISTRY, 2007, 79 (19) :7519-7525

[9] CHARACTERIZATION AND INVITRO TRANSLATION OF POLYADENYLATED MESSENGER RIBONUCLEIC-ACID FROM NEUROSPORA-CRASSA [J].

LUCAS, MC ;

JACOBSON, JW ;

GILES, NH .

JOURNAL OF BACTERIOLOGY, 1977, 130 (03) :1192-1198

[10]

Mohapatra S., 2007, NANOTECHNOLOGY, V18

← 1 2 →