Novel hyaluronan-based nanocarriers for transmucosal delivery of macromolecules

被引：73
作者：
de la Fuente, Maria [1 ]
Seijo, Begona [1 ]
Alonso, Maria J. [1 ]
机构：
[1] Univ Santiago de Compostela, Dept Pharm & Pharmaceut Technol, Fac Pharm, Santiago De Compostela 15782, Spain
来源：
MACROMOLECULAR BIOSCIENCE | 2008年 / 8卷 / 05期
关键词：
chitosan; drug-delivery systems; hyaluronan; macromolecules; nanoparticles;
D O I：
10.1002/mabi.200700190
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The goal of this work was to design a new nanocarrier composed of the glycosaminoglycan hyaluronan and the polysaccharide chitosan, intended for the transmucosal delivery of macromolecules. The nanoparticles were characterized for their size and superficial charge. The incorporation of hyaluronan was verified by agarose gel electrophoresis and Fourier transform infrared (FT-IR) spectroscopy. The ability of the nanosystems to encapsulate macromolecules was studied taking the hydrophilic protein bovine serum albumin (BSA) and the hydrophobic polypeptide Cyclosporine A (CyA) as models. Results showed that the experimental conditions could be conveniently adjusted in order to modulate the physicochemical properties of the carriers and their composition. Moreover, the nanoparticles provided high association efficiencies of the selected macromolecules.
引用
收藏
页码：441 / 450
页数：10
相关论文
共 32 条
  • [1] Recent advances on chitosan-based micro- and nanoparticles in drug delivery
    Agnihotri, SA
    Mallikarjuna, NN
    Aminabhavi, TM
    [J]. JOURNAL OF CONTROLLED RELEASE, 2004, 100 (01) : 5 - 28
  • [2] Nanomedicines for overcoming biological barriers
    Alonso, MJ
    [J]. BIOMEDICINE & PHARMACOTHERAPY, 2004, 58 (03) : 168 - 172
  • [3] ALONSO MJ, 2004, ACS SYM SER, V879, P283
  • [4] Design of poly-ε-caprolactone nanospheres coated with bioadhesive hyaluronic acid for ocular delivery
    Barbault-Foucher, S
    Gref, R
    Russo, P
    Guechot, J
    Bochot, A
    [J]. JOURNAL OF CONTROLLED RELEASE, 2002, 83 (03) : 365 - 375
  • [5] Calvo P, 1997, J APPL POLYM SCI, V63, P125, DOI 10.1002/(SICI)1097-4628(19970103)63:1<125::AID-APP13>3.0.CO
  • [6] 2-4
  • [7] Development of phosphorylated glucomannan-coated chitosan nanoparticles as nanocarriers for protein delivery
    Cuna, M.
    Alonso-Sande, M.
    Remunan-Lopez, G.
    Pivel, J. P.
    Alonso-Lebrero, J. L.
    Alonso, M. J.
    [J]. JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2006, 6 (9-10) : 2887 - 2895
  • [8] Chitosan nanoparticles:: a new vehicle for the improvement of the delivery of drugs to the ocular surface.: Application to cyclosporin A
    De Campos, AM
    Sánchez, A
    Alonso, MJ
    [J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2001, 224 (1-2) : 159 - 168
  • [9] Chitosan-chondroitin sulfate and chitosan-hyaluronate polyelectrolyte complexes: biological properties
    Denuziere, A
    Ferrier, D
    Damour, O
    Domard, A
    [J]. BIOMATERIALS, 1998, 19 (14) : 1275 - 1285
  • [10] Positively charged nanoparticles for improving the oral bioavailability of cyclosporin-A
    El-Shabouri, MH
    [J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2002, 249 (1-2) : 101 - 108
1234