Tissue culture propagation of elite plant of Aloe vera Linn

被引：36
作者：
Aggarwal, D [1 ]
Barna, KS [1 ]
机构：
[1] Thapar Inst Engn & Technol, TIFAC Ctr Relevance & Excellence Agro & Ind Biote, Patiala 147004, Punjab, India
来源：
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY | 2004年 / 13卷 / 01期
关键词：
medicinal plant; Aloe vera; micropropagation; axillary branching;
D O I：
10.1007/BF03263197
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Micropropagation protocol for an elite selection of Aloe vera syn A. barbadensis through enhanced axillary branching was standardized. Murashige and Skoog medium containing 1 mg l(-1) BA and 0.2 mg l(-1) IBA gave highest multiplication. Citric acid at 10 mg l(-1) and liquid medium improved the shoot multiplication. Hundred per cent microshoots produced rooted plantlets within 15 days of culture on hormone-free agar medium. Liquid medium during rooting stage decreased the number of shoots showing rooting response. The plants were successfully transferred in the soil and were morphologically similar to mother plants.
引用
收藏
页码：77 / 79
页数：3
相关论文
共 11 条
  • [1] Micropropagation of the endangered Aloe polyphylla
    Abrie, AL
    van Staden, J
    [J]. PLANT GROWTH REGULATION, 2001, 33 (01) : 19 - 23
  • [2] BARNA KS, 1995, IN VITRO CELL DEV, V33, P44
  • [3] Bhattacharya A. K., 2003, Indian Forester, V129, P93
  • [4] Chaudhuri Sanchita, 2001, Phytomorphology, V51, P155
  • [5] RAPID INVITRO-PROPAGATION OF ALOE-BARBADENSIS MILL
    MEYER, HJ
    VANSTADEN, J
    [J]. PLANT CELL TISSUE AND ORGAN CULTURE, 1991, 26 (03) : 167 - 171
  • [6] MURASHIGE T, 1962, PHYSIOL PLANTARUM, V15, P493, DOI DOI 10.1111/J.1399-3054.1962.TB08052.X
  • [7] INVITRO CULTURE OF ALOE-BARBADENSIS MILL - MICROPROPAGATION FROM VEGETATIVE MERISTEMS
    NATALI, L
    SANCHEZ, IC
    CAVALLINI, A
    [J]. PLANT CELL TISSUE AND ORGAN CULTURE, 1990, 20 (01) : 71 - 74
  • [8] Establishment of Aloe, Gasteria, and Haworthia shoot cultures from inflorescence explants
    Richwine, AM
    Tipton, JL
    Thompson, GA
    [J]. HORTSCIENCE, 1995, 30 (07) : 1443 - 1444
  • [9] INVITRO REGENERATION AND MICROPROPAGATION OF ALOE-VERA L
    ROY, SC
    SARKAR, A
    [J]. SCIENTIA HORTICULTURAE, 1991, 47 (1-2) : 107 - 113
  • [10] INVITRO CULTURE OF ALOE-BARBADENSIS MILL - MORPHOGENETIC ABILITY AND NUCLEAR-DNA CONTENT
    SANCHEZ, IC
    NATALI, L
    CAVALLINI, A
    [J]. PLANT SCIENCE, 1988, 55 (01) : 53 - 59
12