Detection of Cryptosporidium parvum in horses:: Thresholds of acid-fast stain, immunofluorescence assay, and flow cytometry

被引：21
|
作者：
Cole, DJ
Snowden, K
Cohen, ND
Smith, R
机构：
[1] Texas A&M Univ, Coll Vet Med, Dept Large Anim Med & Surg, College Stn, TX 77843 USA
[2] Texas A&M Univ, Coll Vet Med, Dept Vet Pathobiol, College Stn, TX 77843 USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 02期
关键词：
D O I：
10.1128/JCM.37.2.457-460.1999
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Feces collected from three asymptomatic horses and seeded with Cryptosporidium parvum oocysts (10(1) to 10(6)/g of feces) were evaluated by acid-fast staining (AF), an immunofluorescent antibody (IFA) technique, and flow cytometry. The thresholds of detection were 5 x 10(5) oocysts/g of feces for the IFA and AF techniques and 5 x 10(4) oocysts/g for flow cytometry.
引用
收藏
页码：457 / 460
页数：4
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