The human papillomavirus18 E7 protein inhibits CENP-C binding to α-satellite DNA

被引：5

作者：

Yaginuma, Yuji ^{[1
]}

Yoshimoto, Masafumi ^{[1
]}

Eguchi, Ayami ^{[1
]}

Tokuda, Aoi ^{[2
]}

Takahashi, Shoko ^{[2
]}

机构：

[1] Kumamoto Univ, Fac Life Sci, Dept Oncol, Grad Sch Hlth Sci,Chou Ku, Kumamoto 8620976, Japan

[2] Kumamoto Univ, Fac Life Sci, Sch Hlth Sci, Chuou Ku, Kumamoto 8620976, Japan

来源：

VIRUS RESEARCH | 2015年 / 205卷

关键词：

HPV; E7; CENP-C; alpha-Satellite DNA; MITOTIC CHECKPOINT; PCR AMPLIFICATION; CANCER; ANEUPLOIDY; RISK; INACTIVATION; ONCOPROTEINS; CAPACITY; SEQUENCE;

D O I：

10.1016/j.virusres.2015.04.019

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human papillomavirus (HPV) infection leads to aneuploidy, a numerical chromosomal aberration that is caused by dysregulation of chromosomal segregation. We previously found that the E7 proteins of high-risk HPVs, but not of low-risk HPVs, could bind to centromere protein-C (CENP-C). In this study, we first found that CENP-C could bind centromere alpha-satellite DNAs using ChIP analysis and HA-tagged CENP-C/nuc transfected 293T cells. We then investigated if HA-CENP-C/nuc binding to alpha-satellite DNAs was affected by the E7 proteins of high- or low-risk HPVs. We found that transfection of the FLAG tagged HPV18 E7 inhibited the binding of HA-CENP-C/nuc to alpha-satellite DNAs. This finding was confirmed in HeLa S3 cells transfected with siRNA targeted to HPV18 E7 expression. We therefore speculate that altered function of kinetochores as a result of inhibition of CENP-C and alpha-satellite DNAs binding may be associated with the chromosomal abnormalities observed in HPV18-positive cancers. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：27 / 32

页数：6

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