Transposon tools and methods in zebrafish

被引：207

作者：

Kawakami, K ^{[1
]}

机构：

[1] Natl Inst Genet, Div Mol & Dev Biol, Mishima, Shizuoka 4118540, Japan

来源：

DEVELOPMENTAL DYNAMICS | 2005年 / 234卷 / 02期

关键词：

zebrafish; transposon; gene trapping; enhancer trapping; insertional mutagenesis;

D O I：

10.1002/dvdy.20516

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Zebrafish is an excellent model animal to study vertebrate development by genetic approaches. Hundreds of mutations affecting various processes of development have been isolated by chemical mutagenesis and insertional mutagenesis using a pseudotyped retrovirus. However, useful transposon tools and methods had not been available in zebrafish. This is mainly because no active transposable element has been found from the zebrafish genome. Recently, efficient transgenesis, gene trap, and enhancer trap methods have been developed in zebrafish by using the Toll and the Sleeping Beauty transposon systems. These methods should increase the usefulness of zebrafish as a model vertebrate and facilitate the study of developmental biology, genetics, and genomics.

引用

页码：244 / 254

页数：11

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