Development of a bubble-based single cell picking system

被引：0
作者：
Wang, Ronghang [1 ,2 ,3 ,4 ]
Liu, Bingxin [1 ,3 ]
Gong, Jiahao [1 ,3 ]
Zhang, Jinlu [5 ]
Gao, Meng [1 ,3 ]
Zhang, Lunjia [1 ,3 ]
Wang, Xuelin [6 ]
Chen, Sen [1 ,3 ]
Hong, Jie [1 ,3 ]
Gui, Lin [1 ,3 ]
机构：
[1] Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Cryogen, 29 Zhongguancun East Rd, Beijing 10019, Peoples R China
[2] Chinese Acad Sci, Inst Zool, State Key Lab Membrane Biol, Beijing 100101, Peoples R China
[3] Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100039, Peoples R China
[4] Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing 100101, Peoples R China
[5] Peking Univ, Sch Basic Med Sci, Dept Pathol, Beijing Key Lab Tumor Syst Biol,Hlth Sci Ctr, Beijing, Peoples R China
[6] Beihang Univ, Sch Engn Med, Beijing 100191, Peoples R China
来源：
JOURNAL OF MICROMECHANICS AND MICROENGINEERING | 2022年 / 32卷 / 03期
关键词：
single cell picking; bubble-based microfluidics; capillary force; HIGH-THROUGHPUT; TUMOR-CELLS; CAPTURE; QUANTIFICATION; MICROFLUIDICS; ENCAPSULATION; TECHNOLOGIES; CYTOMETRY; TWEEZERS; CHIP;
D O I：
10.1088/1361-6439/ac4c96
中图分类号：
TM [电工技术]; TN [电子技术、通信技术];
学科分类号：
0808 ; 0809 ;
摘要：
In this work, we proposed a novel method to 'pick' single cell from a cluster of cells using bubbles as 'fingers'. Particularly, the bubble was generated in the cell suspension solution via the pores in a porous membrane sandwiched between the solution channel and the gas channel. Controlling the pressure of the gas and the cell suspension could produce a bubble with certain size in the solution channel, and the bubble could capture a cell in its surface due to the interfacial tension between the cell suspension solution and the bubble, and then transfer the cell away. A simplified mechanical model was built to interpret the mechanism of the cell control. This method can be easily applied on multiple fields, including the single cell analysis, drug screening, cells sorting, and tumor biology, since it could separate a single cell from the cell cluster efficiently.
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页数：10
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