Biocompatible Probes Based on Rare-Earth Doped Strontium Aluminates with Long-Lasting Phosphorescent Properties for In Vitro Optical IMAGING

被引:16
作者
Calatayud, David G. [1 ]
Jardiel, Teresa [1 ]
Cordero-Oyonarte, Erica [1 ]
Caballero, Amador C. [1 ]
Villegas, Marina [1 ]
Valle-Noguera, Ana [2 ]
Cruz-Adalia, Aranzazu [2 ]
Peiteado, Marco [1 ]
机构
[1] CSIC, Dept Electroceram, Inst Ceram & Vidrio, Kelsen 5, Madrid 28049, Spain
[2] Univ Complutense Madrid, Sch Med, Octubre Hlth Res Inst Imas12 12, Dept Immunol, Madrid 28040, Spain
关键词
optical bioimaging; fluorescent labels; ceramic composites; phosphorescent inorganics; long persistent luminescence; cellular uptake; NANOPARTICLES; LUMINESCENCE; SRAL2O4EU2+; HYDROLYSIS; DIAGNOSIS; WATER; DY3+; IONS; RED;
D O I
10.3390/ijms23063410
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In recent decades, the demand for biomedical imaging tools has grown very rapidly as a key feature for biomedical research and diagnostic applications. Particularly, fluorescence imaging has gained increased attention as a non-invasive, inexpensive technique that allows real-time imaging. However, tissue auto-fluorescence under external illumination, together with a weak tissue penetration of low wavelength excitation light, largely restricts the application of the technique. Accordingly, new types of fluorescent labels are currently being investigated and, in this search, phosphorescent nanoparticles promise great potential, as they combine the interesting size-dependent properties of nanoscale materials with a long-lasting phosphorescence-type emission that allows optical imaging well after excitation (so avoiding autofluorescence). In this work, core-shell structures consisting of SrAlO:Eu,Dy luminescent cores encapsulated within a biocompatible silica shell were prepared, showing a green persistent phosphorescence with an afterglow time of more than 1000 s. A high-energy ball milling procedure was used to reduce the size of the starting phosphors to a size suitable for cellular uptake, while the silica coating was produced by a reverse micelle methodology that eventually allows the excitation and emission light to pass efficiently through the shell. Confocal fluorescence microscopy using HeLa cancer cells confirmed the potential of the all-ceramic composites produced as feasible labels for in vitro optical imaging.
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页数:16
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