A vertebrate case study of the quality of assemblies derived from next-generation sequences

被引：24

作者：

Ye, Liang ^{[1
]}

Hillier, LaDeana W. ^{[1
]}

Minx, Patrick ^{[1
]}

Thane, Nay ^{[1
]}

Locke, Devin P. ^{[1
]}

Martin, John C. ^{[1
]}

Chen, Lei ^{[1
]}

Mitreva, Makedonka ^{[1
]}

Miller, Jason R. ^{[2
]}

Haub, Kevin V. ^{[1
]}

Dooling, David J. ^{[1
]}

Mardis, Elaine R. ^{[1
]}

Wilson, Richard K. ^{[1
]}

Weinstock, George M. ^{[1
]}

Warren, Wesley C. ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Genome Ctr, St Louis, MO 63108 USA

[2] J Craig Venter Inst, Rockville, MD 20850 USA

来源：

GENOME BIOLOGY | 2011年 / 12卷 / 03期

关键词：

DRAFT ASSEMBLIES; MAMMALIAN GENOMES; ALIGNMENT; READS;

D O I：

10.1186/gb-2011-12-3-r31

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The unparalleled efficiency of next-generation sequencing (NGS) has prompted widespread adoption, but significant problems remain in the use of NGS data for whole genome assembly. We explore the advantages and disadvantages of chicken genome assemblies generated using a variety of sequencing and assembly methodologies. NGS assemblies are equivalent in some ways to a Sanger-based assembly yet deficient in others. Nonetheless, these assemblies are sufficient for the identification of the majority of genes and can reveal novel sequences when compared to existing assembly references.

引用

页数：7

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