Identification and characterization of novel components of a Ca2+/calmodulin-dependent protein kinase cascade in HeLa cells

被引：32
作者：
Ishikawa, Y
Tokumitsu, H
Inuzuka, H
Murata-Hori, M
Hosoya, H
Kobayashi, R
机构：
[1] Kagawa Med Univ, Dept Signal Transduct Sci, Kagawa 7610793, Japan
[2] Hiroshima Univ, Grad Sch Sci, Dept Biol Sci, Higashihiroshima 7398526, Japan
来源：
FEBS LETTERS | 2003年 / 550卷 / 1-3期
关键词：
Ca2+; CaM-kinase cascade; CaM-kinase delta; CaM-K kinase;
D O I：
10.1016/S0014-5793(03)00817-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this report, we cloned a novel calmodulin-kinase (CaM-KIdelta) from HeLa cells and characterized its activation mechanism. CaM-KIdelta exhibits Ca2+/CaM-dependent activity that is enhanced (similar to30-fold) in vitro by phosphorylation of its Thr180 by CaM-K kinase (CaM-KK)alpha consistent with detection of CaM-KIdelta-activating activity in HeLa cells. We also identified a novel CaM-KKbeta isoform (CaM-KKbeta-3) in HeLa cells whose activity was highly Ca2+/CaM-independent. Transiently expressed CaM-KIdelta exhibited enhanced protein kinase activity in HeLa cells without ionomycin stimulation. This sustained activation of CaM-KIdelta was completely abolished by Thr180Ala mutation and inhibited by CaM-KK inhibitor, STO-609, indicating a functional CaM-KK/CaM-KIdelta cascade in HeLa cells. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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收藏
页码：57 / 63
页数：7
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