In vivo involvement of mutated initiation factor IF1 in gene expression control at the translational level

被引：11

作者：

Croitoru, VV ^{[1
]}

Bucheli-Witschel, M ^{[1
]}

Isaksson, LA ^{[1
]}

机构：

[1] Univ Stockholm, Dept Genet Microbiol & Toxicol, S-10691 Stockholm, Sweden

来源：

FEBS LETTERS | 2005年 / 579卷 / 05期

关键词：

+2 codon; IF1; gene expression; codon context; aminoacyl-tRNA acceptor site; Escherichia coli;

D O I：

10.1016/j.febslet.2004.12.072

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence in vivo of mutated forms of translation initiation factor (IF1) on the expression of the lacZ or 3A' reporter genes, with different initiation and/or +2 codons, has been investigated. Reporter gene expression in these infA(IF1) mutants is similar to the wild-type strain. The results do not support the longstanding hypothesis that IF1 could perform discriminatory functions while blocking the aminoacyl-tRNA acceptor site (A-site) of the ribosome. One cold-sensitive IF1 mutant shows a general overexpression, in particular at low temperatures, of both reporter genes at the protein but not mRNA level. (C) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：995 / 1000

页数：6

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