Tissue Engineering to Improve Immature Testicular Tissue and Cell Transplantation Outcomes: One Step Closer to Fertility Restoration for Prepubertal Boys Exposed to Gonadotoxic Treatments

被引:39
作者
Del Vento, Federico [1 ]
Vermeulen, Maxime [1 ]
de Michele, Francesca [1 ,2 ]
Giudice, Maria Grazia [1 ,2 ]
Poels, Jonathan [2 ]
des Rieux, Anne [3 ]
Wyns, Christine [1 ,2 ]
机构
[1] Catholic Univ Louvain, Inst Rech Expt & Clin, Sch Med, Gynecol Androl Unit, B-1200 Brussels, Belgium
[2] Clin Univ St Luc, Dept Gynecol Androl, B-1200 Brussels, Belgium
[3] Catholic Univ Louvain, Louvain Drug Res Inst, Adv Drug Delivery & Biomat Unit, B-1200 Brussels, Belgium
关键词
prepubertal; male fertility; fertility preservation; fertility after cancer; spermatogenesis; testicular tissue; spermatogonial stem cells; transplantation; tissue engineering; nanoparticles; ENDOTHELIAL GROWTH-FACTOR; SPERMATOGENESIS FOLLOWING TRANSPLANTATION; IN-VITRO; GERM-CELLS; SPERMATOGONIAL SURVIVAL; TESTIS TISSUE; EXTRACELLULAR-MATRIX; N-ACETYLCYSTEINE; 3-DIMENSIONAL CULTURE; SEMINIFEROUS TUBULES;
D O I
10.3390/ijms19010286
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Despite their important contribution to the cure of both oncological and benign diseases, gonadotoxic therapies present the risk of a severe impairment of fertility. Sperm cryopreservation is not an option to preserve prepubertal boys' reproductive potential, as their seminiferous tubules only contain spermatogonial stem cells (as diploid precursors of spermatozoa). Cryobanking of human immature testicular tissue (ITT) prior to gonadotoxic therapies is an accepted practice. Evaluation of cryopreserved ITT using xenotransplantation in nude mice showed the survival of a limited proportion of spermatogonia and their ability to proliferate and initiate differentiation. However, complete spermatogenesis could not be achieved in the mouse model. Loss of germ cells after ITT grafting points to the need to optimize the transplantation technique. Tissue engineering, a new branch of science that aims at improving cellular environment using scaffolds and molecules administration, might be an approach for further progress. In this review, after summarizing the lessons learned from human prepubertal testicular germ cells or tissue xenotransplantation experiments, we will focus on the benefits that might be gathered using bioengineering techniques to enhance transplantation outcomes by optimizing early tissue graft revascularization, protecting cells from toxic insults linked to ischemic injury and exploring strategies to promote cellular differentiation.
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页数:24
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