Genotoxicity of hydrated sulfur dioxide on root tips of Allium sativum and Vicia faba

被引:86
作者
Yi, HL [1 ]
Meng, ZQ [1 ]
机构
[1] Shanxi Univ, Sch Life Sci & Technol, Inst Environm Med & Toxicol, Taiyuan 030006, Peoples R China
关键词
genotoxicity; sulfur dioxide; Allium stavium; Vicia faba; micronuclei; pycnosis; anaphase aberration;
D O I
10.1016/S1383-5718(03)00054-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Genotoxicity of sulfur dioxide (SO,) and its hydrates (bisulfite and sulfite) in human lymphocytes and other mammalian cells have been found earlier in our laboratory. In the present studies, we used Allium stavium and Vicia faba cytogenetic tests, which are the highly sensitive and simple plant bioassays. A mixture of sodium bisulfite and sodium sulfite (1:3), at various concentrations from 1 x 10(-4) to 2 x 10(-3) M was used for the treatment. Genotoxicity was expressed in terms of anaphase aberration (AA) frequencies in the Vicia-AA test and in terms of micronuclei (MCN) frequencies in both Vicia-MCN test and Alllium-MCN test. On average, the results showed a 1.7-3.9-fold increase of AA frequencies and a 3.5-4.5-fold increase of MCN frequencies in Vicia root tips as compared with the negative control. Similarly, results of Alllium-MCN test also showed a significant increase in MCN frequencies in the treated samples. In addition, pycnotic cells (PNC) appeared in Allium root tips of treated groups. The frequencies of MCN, AA and PNC increased dose-dependently and the cell cycle delayed at the same time in bisulfite treated samples. Results of the present study suggest that the Vicia and Allium cytogenetic bioassays are efficient. simple and reproducible in genotoxicity studies of bisulfite. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:109 / 114
页数:6
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