MiR-486 Regulates Lactation and Targets the PTEN Gene in Cow Mammary Glands

被引：48

作者：

Li, Dan ^{[1
]}

Xie, Xuejiao ^{[1
]}

Wang, Jie ^{[1
]}

Bian, Yanjie ^{[1
]}

Li, Qingzhang ^{[1
]}

Gao, Xuejun ^{[1
]}

Wang, Chunmei ^{[1
]}

机构：

[1] Northeast Agr Univ, Educ Minist, Key Lab Dairy Sci, Harbin 150030, Heilongjiang, Peoples R China

来源：

PLOS ONE | 2015年 / 10卷 / 03期

关键词：

TUMOR-SUPPRESSOR PTEN; MICRORNA; DIFFERENTIATION; EXPRESSION; PROTEIN; INVOLVEMENT; NETWORKS; BREAST; GROWTH;

D O I：

10.1371/journal.pone.0118284

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mammary gland development is controlled by several genes. Although miRNAs have been implicated in mammary gland function, the mechanism by which miR-486 regulates mammary gland development and lactation remains unclear. We investigated miR-486 expression in cow mammary gland using qRT-PCR and ISH and show that miR-486 expression was higher during the high-quality lactation period. We found that miR-486 targets phosphoinositide signaling in the cow mammary gland by directly downregulating PTEN gene expression and by altering the expression of downstream genes that are important for the function of the mammary gland, such as AKT, mTOR. We analyzed the effect of beta-casein, lactose and triglyceride secretion in bovine mammary gland epithelial cells (BMECs) transfected by an inhibitor and by mimics of miR-486. Our results identify miR-486 as a downstream regulator of PTEN that is required for the development of the cow mammary gland.

引用

页数：16

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