Abundance and diversity of soil petroleum hydrocarbon-degrading microbial communities in oil exploring areas

被引：87
作者：
Yang, Yuyin [1 ]
Wang, Jie [1 ]
Liao, Jingqiu [1 ]
Xie, Shuguang [1 ]
Huang, Yi [1 ]
机构：
[1] Peking Univ, Coll Environm Sci & Engn, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100871, Peoples R China
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2015年 / 99卷 / 04期
关键词：
Alkane; Biodegradation; alkB; nah; Geographic location; Oil exploring area; Petroleum hydrocarbon; Polycyclic aromatic hydrocarbons (PAHs); BACTERIAL COMMUNITIES; DIOXYGENASE GENES; CATABOLIC GENES; BIODEGRADATION; BIOREMEDIATION; DEGRADATION; PAH; MICROORGANISMS; SEDIMENTS; CONTAMINATION;
D O I：
10.1007/s00253-014-6074-z
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Alkanes and polycyclic aromatic hydrocarbons (PAHs) are the commonly detected petroleum hydrocarbon contaminants in soils in oil exploring areas. Hydrocarbon-degrading genes are useful biomarks for estimation of the bioremediation potential of contaminated sites. However, the links between environmental factors and the distribution of alkane and PAH metabolic genes still remain largely unclear. The present study investigated the abundances and diversities of soil n-alkane and PAH-degrading bacterial communities targeting both alkB and nah genes in two oil exploring areas at different geographic regions. A large variation in the abundances and diversities of alkB and nah genes occurred in the studied soil samples. Various environmental variables regulated the spatial distribution of soil alkane and PAH metabolic genes, dependent on geographic location. The soil alkane-degrading bacterial communities in oil exploring areas mainly consisted of Pedobacter, Mycobacterium, and unknown alkB-harboring microorganisms. Moreover, the novel PAHdegraders predominated in nah gene clone libraries from soils of the two oil exploring areas. This work could provide some new insights towards the distribution of hydrocarbon-degrading microorganisms and their biodegradation potential in soil ecosystems.
引用
收藏
页码：1935 / 1946
页数：12
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