Recent Advances in Aptamer-Based Nanopore Sensing at Single-Molecule Resolution

被引：6

作者：

Lv, Pengrui ^{[1
]}

Zhang, Wenxin ^{[1
]}

Yang, Yongyi ^{[1
]}

Gao, Huilin ^{[1
]}

Li, Shuang ^{[1
,2
]}

Tan, Cherie S. ^{[1
,2
]}

Ming, Dong ^{[1
,2
,3
]}

机构：

[1] Tianjin Univ, Acad Med Engn & Translat Med, Tianjin 300072, Peoples R China

[2] Tianjin Key Lab Brain Sci & Neuroengn, Tianjin 300072, Peoples R China

[3] Tianjin Univ, Dept Biomed Engn, Tianjin 300072, Peoples R China

来源：

CHEMISTRY-AN ASIAN JOURNAL | 2022年 / 17卷 / 16期

基金：

中国国家自然科学基金;

关键词：

aptamer; electrochemistry; nanopore; single-molecule; STAPHYLOCOCCAL ALPHA-HEMOLYSIN; SOLID-STATE NANOPORES; SENSITIVE DETECTION; AEROLYSIN NANOPORE; DNA TRANSLOCATION; CRYSTAL-STRUCTURE; GAMMA-HEMOLYSIN; OMPG NANOPORE; IN-VITRO; PROTEIN;

D O I：

10.1002/asia.202200364

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Aptamers as the recognition element of the stochastic nanopore sensors have been under intense investigation. This paper reviews recent research advances in aptamer-based nanopore sensing techniques, including the classification and selection of nanopores (biological nanopores, solid-state nanopores, and nanopipettes), different strategies of aptamer-based nanopore sensing, and their values and outlook for applications in areas such as environmental analysis, precision diagnosis, pharmaceutical industry, and security. Furthermore, the single-molecule nanopore sensors have been applied to reveal the aptamer-target interactions, such as recognition orientation, binding sites, and conformational heterogeneity dissociation kinetics at the single-molecule level. In this review, recent research efforts to develop aptamer-based single-molecule nanopore sensors with high selectivity and sensitivity are highlighted, and some perspectives are drawn.

引用

页数：13

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