Reliability of human cortical organoid generation

被引：309

作者：

Yoon, Se-Jin ^{[1
]}

Elahi, Lubayna S. ^{[1
]}

Pasca, Anca M. ^{[2
]}

Marton, Rebecca M. ^{[1
]}

Gordon, Aaron ^{[3
]}

Revah, Omer ^{[1
]}

Miura, Yuki ^{[1
]}

Walczak, Elisabeth M. ^{[4
]}

Holdgate, Gwendolyn M. ^{[4
]}

Fan, H. Christina ^{[4
]}

Huguenard, John R. ^{[5
]}

Geschwind, Daniel H. ^{[3
,6
]}

Pasca, Sergiu P. ^{[1
,7
]}

机构：

[1] Stanford Univ, Dept Psychiat & Behav Sci, Sch Med, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Pediat, Div Neonatol, Sch Med, Stanford, CA 94305 USA

[3] Univ Calif Los Angeles, David Geffen Sch Med, Dept Neurol, Los Angeles, CA 90095 USA

[4] BD Biosci, Menlo Pk, CA USA

[5] Stanford Univ, Dept Neurol & Neurol Sci, Sch Med, Stanford, CA 94305 USA

[6] Univ Calif Los Angeles, David Geffen Sch Med, Dept Human Genet, Los Angeles, CA 90095 USA

[7] Stanford Univ, Human Brain Organogenesis Program, Stanford, CA 94305 USA

来源：

NATURE METHODS | 2019年 / 16卷 / 01期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

FRAMEWORK;

D O I：

10.1038/s41592-018-0255-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The differentiation of pluripotent stem cells in three-dimensional cultures can recapitulate key aspects of brain development, but protocols are prone to variable results. Here we differentiated multiple human pluripotent stem cell lines for over 100 d using our previously developed approach to generate brain-region-specific organoids called cortical spheroids and, using several assays, found that spheroid generation was highly reliable and consistent. We anticipate the use of this approach for large-scale differentiation experiments and disease modeling.

引用

页码：75 / +

页数：6

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