Effect of matrine on lipopolysaccharides/D-galactosamine-induced hepatitis and tumor necrosis factor release from macrophages in vitro

被引:0
作者
Hu, ZL
Zhang, JP
Yu, XB
Lin, W
Qian, DH
Wan, MB
机构
[1] Second Mil Med Univ, COLL PHARM, SHANGHAI 200433, PEOPLES R CHINA
[2] Second Mil Med Univ, CHANGHAI HOSP, DEPT INFECT DIS, SHANGHAI 200433, PEOPLES R CHINA
关键词
matrine; toxic hepatitis; galactosamine; lipopolysaccharides; tumor necrosis factor; peritoneal macrophages;
D O I
暂无
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
To study the effects of matrine (Mat) on lipopolysaccharides (LPS)-induced fatal hepatitis in D-galactosamine (D-GalN)-sensitized mice and tumor necrosis factor (TNF) release from peritoneal macrophages (PMO). METHODS: Mice were pretreated with Mat (10, 50 mg . kg(-1), ip, bid x 3 d), and then;injected ip LPS + D-GalN. Liver injury was assessed by quantifying plasma activity of alanine aminotransferase (ALT) and histopathological examination. The TNF activities in the; supernatants of mouse PMO stimulated with LPS in the presence of Mat (32.5 - 500 mg . L(-1)) were monitored by the L929 target cells lytic assay. RESULTS: Mat pretreatment markedly diminished hepatic injury induced by LPS in combination with D-GalN. Mat inhibited LPS-induced TNF release from mouse PMO in vitro in a concentration-dependent manner. CONCLUSION: Mat protected the D-GalN-treated mice from the development of fatal hepatitis induced by LPS, and inhibited the LPS-induced TNF release from mouse PMO.
引用
收藏
页码:351 / +
相关论文
共 6 条
  • [1] INVOLVEMENT OF TUMOR-NECROSIS-FACTOR-ALPHA IN DEVELOPMENT OF HEPATIC-INJURY IN GALACTOSAMINE-SENSITIZED MICE
    HISHINUMA, I
    NAGAKAWA, J
    HIROTA, K
    MIYAMOTO, K
    TSUKIDATE, K
    YAMANAKA, T
    KATAYAMA, K
    YAMATSU, I
    [J]. HEPATOLOGY, 1990, 12 (05) : 1187 - 1191
  • [2] HU ZL, 1993, ACTA PHARMACOL SIN, V14, P550
  • [3] TRANSAMINASE ACTIVITY IN HUMAN BLOOD
    KARMEN, A
    WROBLEWSKI, F
    LADUE, JS
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1955, 34 (01) : 126 - 133
  • [4] LETHAL TOXICITY OF LIPOPOLYSACCHARIDE AND TUMOR-NECROSIS-FACTOR IN NORMAL AND D-GALACTOSAMINE-TREATED MICE
    LEHMANN, V
    FREUDENBERG, MA
    GALANOS, C
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1987, 165 (03) : 657 - 663
  • [5] Tao S. C., 1992, CHIN PHARM J, V27, P201
  • [6] TUMOR NECROSIS FACTOR IS A TERMINAL MEDIATOR IN GALACTOSAMINE ENDOTOXIN-INDUCED HEPATITIS IN MICE
    TIEGS, G
    WOLTER, M
    WENDEL, A
    [J]. BIOCHEMICAL PHARMACOLOGY, 1989, 38 (04) : 627 - 631