A fluorescent Arg-Gly-Asp (RGD) peptide-naphthalenediimide (NDI) conjugate for imaging integrin αvβ3 in vitro

被引：22
|
作者：
Hu, Zhiyuan [1 ,2 ]
Arrowsmith, Rory L. [2 ]
Tyson, James A. [2 ]
Mirabello, Vincenzo [2 ]
Ge, Haobo [2 ]
Eggleston, Ian M. [3 ]
Botchway, Stanley W. [4 ]
Pantos, G. Dan [2 ]
Pascu, Sofia I. [2 ]
机构：
[1] China Univ Petr, State Key Lab Heavy Oil Proc, Beijing 102249, Peoples R China
[2] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England
[3] Univ Bath, Dept Pharm & Pharmacol, Claverton Down BA2 7AY, Avon, England
[4] Rutherford Appleton Lab, Cent Laser Facil, STFC, Didcot OX11 0QX, Oxon, England
来源：
CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 32期
基金：
英国工程与自然科学研究理事会;
关键词：
TUMOR ANGIOGENESIS; CELL-ADHESION; DESIGN;
D O I：
10.1039/c4cc08265f
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI-RGDfK in cancer cells.
引用
收藏
页码：6901 / 6904
页数：4
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