Design, synthesis, and evaluation of a new fluorescent probe for measuring polymyxin-lipopolysaccharide binding interactions

Fluorescence assays employing semisynthetic or commercial dansyl-polymyxin B have been widely employed to assess the affinity of polycations, including polymyxins, for bacterial cells and lipopolysaccharide (LPS). The five primary gamma-amines on diaminobutyric acid residues of polymyxin B are potentially derivatized with dansyl-chloride. Mass spectrometric analysis of the commercial product revealed a complex mixture of di- or tetra-dansyl-substituted polymyxin B. We synthesized a mono-substituted fluorescent derivative, dansyl[Lys](1)polymyxin B-3. The affinity of polymyxin for purified gram-negative LPS and whole bacterial cells was investigated. The affinity of dansyl[Lys](1)polymyxin B-3 for LPS was comparable to polymyxin B and colistin, and considerably greater (K-d < 1 mu M) than for whole cells (K-d similar to 6-12 mu M). Isothermal titration calorimetric studies demonstrated exothermic enthalpically driven binding between both polymyxin B and dansyl[Lys](1)polymyxin B-3 to LPS, attributed to electrostatic interactions. The hydrophobic dansyl moiety imparted a greater entropic contribution to the dansyl[Lys](1)polymyxin B-3-LPS reaction. Molecular modeling revealed a loss of electrostatic contact within the dansyl[Lys](1)polymyxin B-3-LPS complex due to steric hindrance from the dansyl[Lys](1) fluorophore; this corresponded with diminished antibacterial activity (MIC >= 16 mu g/mL). Dansyl[Lys](1)polymyxin B-3 may prove useful as a screening tool for drug development. (C) 2010 Elsevier Inc. All rights reserved.