Lipase-mediated acidolysis of butteroil with free conjugated linoleic acid in a packed bed reactor

被引：7

作者：

Sehanputri, PS ^{[1
]}

Hill, CG ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Chem Engn, Madison, WI 53706 USA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 2003年 / 83卷 / 05期

关键词：

Candida antarctica lipase; butteroil; acidolysis; conjugated linoleic acid (CLA); packed bed reactor; immobilized lipase;

D O I：

10.1002/bit.10720

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Acidolysis of butteroil with free conjugated linoleic acid (CLA) was studied in a packed bed reactor containing an immobilized Candida antarctica (fraction B) lipase. Kinetic data were used to develop quantitative reversible rate expressions of the general Michaelis-Menten form that also incorporate a term for first-order deactivation of the enzyme. The extent of incorporation of CLA in the triacylglycerols of butteroil was characterized for reactions carried out at several temperatures (namely 45degrees, 50degrees, and 55degreesC) with different weight ratios of butteroil to CLA (namely 10:1 and 2:1). At the optimum operating temperature of 50degreesC, similar levels of incorporation of CLA (60% to 85%) were achieved at low space times (<3 h) for both 10:1 and 2:1 (w/w) ratios of butteroil to CLA. (C) 2003 Wiley Periodicals, Inc.

引用

页码：608 / 617

页数：10

共 18 条

[1]

Box GEP., 1978, Statistics for experimenters

[2]

Chin S. F., 1992, Journal of Food Composition and Analysis, V5, P185, DOI 10.1016/0889-1575(92)90037-K

[3]

DOYLE E, 1998, INFORM, V9, P69

[4] Continuous interesterification of butteroil and conjugated linoleic acid in a tubular reactor packed with an immobilized lipase [J].

Garcia, HS ;

Keough, KJ ;

Arcos, JA ;

Hill, CG .

BIOTECHNOLOGY TECHNIQUES, 1999, 13 (06) :369-373

[5]

HA YL, 1990, CANCER RES, V50, P1097

[6] KINETIC MODELING OF INTERESTERIFICATION REACTIONS CATALYZED BY IMMOBILIZED LIPASE [J].

REYES, HR ;

HILL, CG .

BIOTECHNOLOGY AND BIOENGINEERING, 1994, 43 (02) :171-182

[7]

SCIMECA JA, 1994, ADV EXP MED BIOL, V364, P59

[8]

Sehanputri PS, 1999, BIOTECHNOL BIOENG, V64, P568, DOI 10.1002/(SICI)1097-0290(19990905)64:5<568::AID-BIT7>3.0.CO

[9]

2-E

[10]

Sehanputri PS, 2000, BIOTECHNOL BIOENG, V69, P450, DOI 10.1002/1097-0290(20000820)69:4<450::AID-BIT11>3.3.CO

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