Intestinal D-galactose transport in an endotoxemia model in the rabbit

被引:30
作者
Amador, P.
Garcia-Herrera, J.
Marca, M. C.
de la Osada, J.
Acin, S.
Navarro, M. A.
Salvador, M. T.
Lostao, M. P.
Rodriguez-Yoldi, M. J.
机构
[1] Univ Zaragoza, Fac Vet, Dept Pharmacol & Physiol, Physiol Unit, E-50013 Zaragoza, Spain
[2] Univ Zaragoza, Fac Vet, Dept Biochem & Mol Biol, Biochem Unit, E-50013 Zaragoza, Spain
[3] Univ Navarra, Dept Physiol & Nutr, E-31008 Pamplona, Spain
[4] Univ Zaragoza, Fac Vet, Dept Anim Pathol, Internal Med Unit, E-50013 Zaragoza, Spain
关键词
lipopolysaccharide; mitogen-activated protein kinase; protein kinase A; protein kinase C; Proteasome; Na+/glucose cotransporter 1;
D O I
10.1007/s00232-007-9012-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lipopolysaccharide ( LPS) is an endotoxin causing sepsis. Studies from our laboratory revealed impaired intestinal absorption of L- leucine and D- fructose in LPS- treated rabbits. The aim of this study was to examine intestinal D- galactose transport following intravenous administration of LPS in the rabbit and to identify the cellular mechanisms driving this process. Endotoxin treatment diminished the buildup of D- galactose in intestinal tissue, the mucosal to serosal transepithelial flux of the sugar and its uptake by brush border membrane vesicles ( BBMVs). Intracellular signaling pathways associated with protein kinase C ( PKC), protein kinase A ( PKA), p38 mitogen- activated protein kinase ( p38MAPK), Jun N- terminal kinase ( JNK), MAPK/ extracellular signalregulated kinases 1 and 2 ( MEK1/ 2) and proteasome were found to be involved in this reduction in sugar uptake. Na+/ glucose cotransporter 1 ( SGLT1) protein levels in BBMVs were lower for LPS- treated animals than control animals. These findings indicate that LPS inhibits the intestinal absorption of D- galactose via a complex cellular mechanism that could involve posttranscriptional regulation of the SGLT1 transporter.
引用
收藏
页码:125 / 133
页数:9
相关论文
共 49 条
[31]   Up-regulation of the proteasome subunit LMP7 in tissues of endotoxemic rats [J].
Nelson, JE ;
Loukissa, A ;
Altschuller-Felberg, C ;
Monaco, JJ ;
Fallon, JT ;
Cardozo, C .
JOURNAL OF LABORATORY AND CLINICAL MEDICINE, 2000, 135 (04) :324-331
[32]   Activation of NF-κB varies in different regions of the gastrointestinal tract during endotoxemia [J].
Pritts, TA ;
Moon, MR ;
Wang, Q ;
Hungness, ES ;
Salzman, AL ;
Fischer, JE ;
Hasselgren, PO .
SHOCK, 2000, 14 (02) :118-122
[33]   NA+-STIMULATED ATPASE ACTIVITIES IN KIDNEY BASAL-LATERAL PLASMA-MEMBRANES [J].
PROVERBIO, F ;
DELCASTILLO, JR .
BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 646 (01) :99-108
[34]   INHIBITORS OF THE PROTEASOME BLOCK THE DEGRADATION OF MOST CELL-PROTEINS AND THE GENERATION OF PEPTIDES PRESENTED ON MHC CLASS-I MOLECULES [J].
ROCK, KL ;
GRAMM, C ;
ROTHSTEIN, L ;
CLARK, K ;
STEIN, R ;
DICK, L ;
HWANG, D ;
GOLDBERG, AL .
CELL, 1994, 78 (05) :761-771
[35]   Yersinia enterocolitica impairs activation of transcription factor NF-κB:: Involvement in the induction of programmed cell death and in the suppression of the macrophage tumor necrosis factor α production [J].
Ruckdeschel, K ;
Harb, S ;
Roggenkamp, A ;
Hornef, M ;
Zumbihl, R ;
Köhler, S ;
Heesemann, J ;
Rouot, B .
JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 187 (07) :1069-1079
[36]   BRUSH-BORDER TRANSPORT OF GLUTAMINE AND OTHER SUBSTRATES DURING SEPSIS AND ENDOTOXEMIA [J].
SALLOUM, RM ;
COPELAND, EM ;
SOUBA, WW .
ANNALS OF SURGERY, 1991, 213 (05) :401-410
[37]   Regulation of NF-κB activation by MAP kinase cascades [J].
Schulze-Osthoff, K ;
Ferrari, D ;
Riehemann, K ;
Wesselborg, S .
IMMUNOBIOLOGY, 1997, 198 (1-3) :35-49
[38]   TNF ACTIVATES NF-KAPPA-B BY PHOSPHATIDYLCHOLINE-SPECIFIC PHOSPHOLIPASE-C-INDUCED ACIDIC SPHINGOMYELIN BREAKDOWN [J].
SCHUTZE, S ;
POTTHOFF, K ;
MACHLEIDT, T ;
BERKOVIC, D ;
WIEGMANN, K ;
KRONKE, M .
CELL, 1992, 71 (05) :765-776
[39]   PREPARATION AND PROPERTIES OF BRUSH-BORDER MEMBRANE-VESICLES FROM HUMAN SMALL-INTESTINE [J].
SHIRAZIBEECHEY, SP ;
DAVIES, AG ;
TEBBUTT, K ;
DYER, J ;
ELLIS, A ;
TAYLOR, CJ ;
FAIRCLOUGH, P ;
BEECHEY, RB .
GASTROENTEROLOGY, 1990, 98 (03) :676-685
[40]   SEPSIS IMPAIRS GUT AMINO-ACID-ABSORPTION [J].
SODEYAMA, M ;
GARDINER, KR ;
REGAN, MC ;
KIRK, SJ ;
EFRON, G ;
BARBUL, A .
AMERICAN JOURNAL OF SURGERY, 1993, 165 (01) :150-154