Lymphocyte DNA damage in patients with acute coronary syndrome and its relationship with severity of acute coronary syndrome

Objective: The aim of this study was to investigate the association between lymphocyte DNA damage and acute coronary syndromes (ACS). Methods: The study population contained 53 patients with ACS, 48 patients with stable angina and 35 voluntary healty subjects. DNA damage was assessed by alkaline comed assay in peripheral lymphocyte and plasma levels of total antioxidant capacity (TAC) were determined using a novel automated measurement method. Results: In ACS patients, DNA damage was significantly higher than in patients with stable angina and control subjects (144 +/- 52 AU, 116 +/- 37, 68 +/- 34 AU; for three p < 0.001, respectively). The TAC levels in patients with ACS were lower than the other groups (1.24 +/- 0.31 mmol Trolox equiv./l, 1.46 +/- 0.29 mmol Trolox equiv./l, p < 0.05, respectively). DNA damage values in patients with acute miyocardial infarction were significantly higher than in patients with unstable angina (159.8 +/- 53.0 AU versus 131.8 +/- 48.4 AU; p < 0.05, respectively). Lymphocyte DNA damage values in patients with ACS showed positive correlation with D-dimer (r = 0.880, p < 0.001) troponin I (r = 538, p < 0.001) and C-reactive protein (r = 0.544, p < 0.001) and negative correlation with TAC (r = -0.346, p = 0.011). In multiple linear regression analysis, TAC (beta= -0.213, p = 0.001) and D-dimer (P = 0.697, p < 0.001) were independent predictors of DNA damage in patients with ACS. Conclusions: These findings indicate that lymphocyte DNA damage level increases in patients with ACS. Elevated DNA damage may be related with plaque instability and be useful for the identification of patients with acute coronary syndromes. (C) 2005 Elsevier B.V. All rights reserved.