Chemiluminescence flow-through biosensor for glucose with eggshell membrane as enzyme immobilization platform

被引：61
作者：
Li, Baoxin [1 ]
Lan, Dan [1 ]
Zhang, Zhujun [1 ]
机构：
[1] Shaanxi Normal Univ, Sch Chem & Mat Sci, Key Lab Anal Chem Life Sci Shaanxi Prov, Xian 710062, Peoples R China
来源：
ANALYTICAL BIOCHEMISTRY | 2008年 / 374卷 / 01期
基金：
中国国家自然科学基金;
关键词：
chemiluminescence; flow-through biosensor; eggshell membrane; glucose;
D O I：
10.1016/j.ab.2007.10.036
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study, a new chemiluminescence (CL) flow-through biosensor for glucose was developed by immobilizing glucose oxidase (GOD) and horseradish peroxidase (HRP) on the eggshell membrane with glutaraldehyde as a cross-linker. The CL detection involved enzymatic oxidation Of glucose to D-gluconic acid and hydrogen peroxide (H2O2) and then H2O2 oxidizing luminol to produce CL emission in the presence of HRP. The immobilization condition (e.g., immobilization time, GOD/HRP ratio, glutaraldehyde concentration) was studied in detail. It showed good storage stability at 4 degrees C over a 5-month period. The proposed biosensor exhibited short response time, high sensitivity, easy operation, and simple sensor assembly, and the proposed biosensor was successfully applied to the determination of glucose in human serum. (c) 2007 Elsevier Inc. All rights reserved.
引用
收藏
页码：64 / 70
页数：7
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