Tk-deleted pseudorabies virus retains high pathogenicity in rats

被引：11

作者：

Zhang, Lirong ^{[1
]}

Ruan, Keyue ^{[1
]}

Sang, Guoju ^{[1
]}

Xu, Zhaoyang ^{[1
]}

Tong, Wu ^{[1
]}

Yu, Hai ^{[1
]}

Shan, Tongling ^{[1
]}

Gao, Fei ^{[1
]}

Li, Liwei ^{[1
]}

Kong, Ning ^{[1
]}

Tong, Guangzhi ^{[1
,2
]}

Zheng, Hao ^{[1
,2
]}

机构：

[1] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Shanghai 200241, Peoples R China

[2] Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

来源：

JOURNAL OF VETERINARY RESEARCH | 2021年 / 65卷 / 04期

关键词：

pseudorabies virus; thymidine kinase; rat; PATHOGENESIS; VACCINE; MUTANT; DNA;

D O I：

10.2478/jvetres-2021-0056

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Introduction The pseudorabies virus (PRV) gene encoding thymidine kinase (tk) is an important virulence-associated factor. Attenuation of PRV in susceptible animals is a frequent result of tk deletion. The aim of the study was to assess the pathogenicity of tk-deleted PRV in rats. Material and Methods Sprague Dawley rats were infected with the tk-deleted PRV strain SuHV-1 Delta TK:247via intranasal or intramuscular inoculation. PRV loads in ten tissues from dead and euthanised rats were determined using real-time PCR. Results Infection with SuHV-1 Delta TK:247 could cause death in rats. The 50% lethal dose (LD50) of SuHV-1 Delta TK:247 via intranasal inoculation was 10(3.16) TCID50 in rats. Intramuscular inoculation required a higher dose of SuHV-1 Delta TK:247 (10(5.0) TCID50). A high SuHV-1 Delta TK:247 titre was observed in the trigeminal ganglia or spinal cord of dead rats. Conclusion The results of this study show that rats are highly susceptible to PRV infection, and tk deletion did not completely diminish the pathogenicity of PRV in rats.

引用

页码：401 / 405

页数：5

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