Immuno-affinity Amperometric Detection of Bacterial Infections

被引：27

作者：

Zhu, Yingdi ^{[1
]}

Jovic, Milica ^{[1
]}

Lesch, Andreas ^{[1
]}

Lovey, Lysiane Tissieres ^{[2
]}

Prudent, Michel ^{[3
]}

Pick, Horst ^{[1
]}

Girault, Hubert H. ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Dept Chem & Chem Engn, CH-1951 Sion, Switzerland

[2] Hop Valais, ICH, CH-1951 Sion, Switzerland

[3] Univ Lausanne, Lab Rech Prod Sanguins, Transfus Interreg CRS, Fac Biol & Med, CH-1066 Lausanne, Switzerland

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2018年 / 57卷 / 45期

关键词：

bacterial infections; biosensors; electrochemistry; metabolic activity; point-of-care testing; CARBON NANOTUBES ELECTRODES; BLOOD-STREAM INFECTIONS; TETRAZOLIUM SALTS; PLASMA-MEMBRANE; DIAGNOSIS; RESAZURIN; TOOLS;

D O I：

10.1002/anie.201808666

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A combination of an immuno-affinity enrichment strategy and sensitive amperometric read-out was implemented in a point-of-care platform intended for bacterial infection analysis. Bacterial cells, selectively captured and enriched from complex matrices through immuno-affinity, were detected by amperometric monitoring of the redox state of metabolic activity indicators, providing species identification and viable-cell quantification. The method was successfully employed for the diagnosis of bacterial infections including antimicrobial susceptibility testing with only several hours of total working time.

引用

页码：14942 / 14946

页数：5

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