O-specific polysaccharides structures of Pseudomonas strains isolated from the strawberry leaves

被引:2
作者
Zdorovenko, Evelina L. [1 ]
Kadykova, Alexandra A. [1 ,4 ]
Shashkov, Alexander S. [1 ]
Kiseleva, Elena P. [2 ]
Savich, Victoria V. [3 ]
Novik, Galina, I [3 ]
机构
[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Leninsky Prospekt 47, Moscow 119991, Russia
[2] Natl Acad Sci Belarus, Inst Bioorgan Chem, Minsk 220141, BELARUS
[3] Natl Acad Sci Belarus, Inst Microbiol, Minsk 220141, BELARUS
[4] DI Mendeleev Univ Chem Technol Russia, Russian Acad Sci, Higher Chem Coll, Moscow, Russia
关键词
Pseudomonas koreensis; Pseudomonas oryzihabitans; O-polysaccharide structure; NMR; MECHANISMS;
D O I
10.1016/j.carres.2020.107932
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two Pseudomonas strains were isolated from the strawberry leaves. The O-antigens were obtained using phenolwater method and mild acid degradation. The following structures of the O-polysaccharides were established by sugar analysis and 2D NMR spectroscopy: OPS of Pseudomonas koreensis BIM B-970G -> 3)-alpha-D-FucNAcp-(1 -> 2)-beta-D-Quip3NAc-(1 -> 3)-alpha-L-6dTalp4OAc-(1 -> OPS of Pseudomonas oryzihabitans BIM B-1072G -> 4)-alpha-L-FucpNAm3OAc-(1 -> 3)-alpha-D-QuipNAc (1 -> 4)-beta-D-GlcpNAc3NAcA-(1 -> Where Am - acetimidoyl.
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页数:5
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