Dendrimeric biosensor for detection of E. coli O157:H7 in diet

The focus of present research work was to ensure the safety of variety of products like milk, bread, ice-cream, curd, etc. by detecting harmful pathogen i.e. E. coli O157:H7. This study evaluated the presence of E. coli O157:H7 in fresh food and water. Food samples and water were artificially contaminated with the known concentration of E. coli O157:H7 and the concentration was estimated after 8 and 12h. Estimation of E. coli O157:H7 was determined by adding prepared dendrimeric biosensors to detect E. coil O157 using ELISA 96 Wells and the end point was estimated by measuring the pH change. The prepared biosensors showed the limit of detection in range of 101-10-8 CFU/mL. Estimated detection time for detecting E. coli O157:H7 from diet was found to be 2h. It is concluded that modern biosensor-based method for estimating E. coli O157:H7 in diet is sensitive, specific and precise.