Lipid membranes supported on optically transparent nanosilicas: Synthesis and application in characterization of protein-membrane interactions

被引:3
|
作者
Fadeev, Alexander Y. [1 ]
DeGrado, William F. [2 ]
机构
[1] Seton Hall Univ, Dept Chem & Biochem, S Orange, NJ 07079 USA
[2] Univ Penn, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
基金
美国国家科学基金会;
关键词
Lipid membrane; Silica nanoparticles; Protein-membrane interactions; PHOSPHOLIPID-BILAYERS; MONOLAYERS; PARTICLES; SILICA;
D O I
10.1016/j.jcis.2010.11.064
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
We describe methods of preparation of lipid membranes supported on optically transparent nanosilicas (20 and 30 nm diameter) and an initial application of these materials to characterize membrane-protein binding using conventional circular dichroism and fluorescence spectroscopy. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:265 / 268
页数:4
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