Lanthanide-Binding Tags for 3D X-ray Imaging of Proteins in Cells at Nanoscale Resolution

被引：37

作者：

Victor, Tiffany W. ^{[1
,2
]}

O'Toole, Katherine H. ^{[3
]}

Easthon, Lindsey M. ^{[3
]}

Ge, Mingyuan ^{[1
]}

Smith, Randy J. ^{[1
]}

Huang, Xiaojing ^{[1
]}

Yan, Hanfei ^{[1
]}

Chu, Yong S. ^{[1
]}

Chen, Si

Gursoy, Doga ^{[4
,5
]}

Ralle, Martina ^{[6
]}

Imperiali, Barbara ^{[7
]}

Allen, Karen N. ^{[3
]}

Miller, Lisa M. ^{[1
,2
]}

机构：

[1] Brookhaven Natl Lab, Natl Synchrotron Light Source 2, Upton, NY 11973 USA

[2] SUNY Stony Brook, Dept Chem, Stony Brook, NY 11794 USA

[3] Boston Univ, Dept Chem, Boston, MA 02215 USA

[4] Argonne Natl Lab, Adv Photon Source, Lemont, IL 60439 USA

[5] Northwestern Univ, Dept Elect Engn & Comp Sci, Evanston, IL 60298 USA

[6] Oregon Hlth & Sci Univ, Dept Mol & Med Genet, Portland, OR 97239 USA

[7] MIT, Dept Biol, Cambridge, MA 02139 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2020年 / 142卷 / 05期

基金：

美国能源部; 美国国家卫生研究院;

关键词：

PROTEASOME PATHWAY; OMPA; MICROSCOPY; PEPTIDES; DISEASE; ORIGIN;

D O I：

10.1021/jacs.9b11571

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the application of lanthanide-binding tags (LBTs) for two- and three-dimensional X-ray imaging of individual proteins in cells with a sub-15 nm beam. The method combines encoded LBTs, which are tags of minimal size (ca. 15-20 amino acids) affording high-affinity lanthanide ion binding, and X-ray fluorescence microscopy (XFM). This approach enables visualization of LBT-tagged proteins while simultaneously measuring the elemental distribution in cells at a spatial resolution necessary for visualizing cell membranes and eukaryotic subcellular organelles.

引用

页码：2145 / 2149

页数：5

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