Molecular architecture of the complete COG tethering complex

被引：33
作者：
Ha, Jun Yong [1 ]
Chou, Hui-Ting [2 ]
Ungar, Daniel [1 ,3 ]
Yip, Calvin K. [2 ,4 ]
Walz, Thomas [2 ,5 ]
Hughson, Frederick M. [1 ]
机构：
[1] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA
[2] Harvard Med Sch, Dept Cell Biol, Boston, MA USA
[3] Univ York, Dept Biol, York, N Yorkshire, England
[4] Univ British Columbia, Dept Biochem & Mol Biol, Vancouver, BC, Canada
[5] Rockefeller Univ, Lab Mol Electron Microscopy, 1230 York Ave, New York, NY 10021 USA
来源：
NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2016年 / 23卷 / 08期
基金：
英国生物技术与生命科学研究理事会;
关键词：
STRUCTURAL BASIS; GARP COMPLEX; GOLGI; SUBUNIT; GLYCOSYLATION; DEFICIENCY; MUTATION; REVEALS;
D O I：
10.1038/nsmb.3263
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The conserved oligomeric Golgi (COG) complex orchestrates vesicular trafficking to and within the Golgi apparatus. Here, we use negative-stain electron microscopy to elucidate the architecture of the hetero-octameric COG complex from Saccharomyces cerevisiae. Intact COG has an intricate shape, with four (or possibly five) flexible legs, that differs strikingly from that of the exocyst complex and appears to be well suited for vesicle capture and fusion.
引用
收藏
页码：758 / +
页数：4
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