Characterization of Cecal microbiota and response to an orally administered Lactobacillus Probiotic strain in the broiler chicken

被引:35
作者
Gerard, Philippe
Brezillon, Christophe
Quere, Florence
Salmon, Annick
Rabot, Sylvie
机构
[1] INRA, Unite Ecol & Physiol Syst Digestif, FR-78352 Jouy En Josas, France
[2] Primex SAS, Languidic, France
关键词
fluorescent in situ hybridization; temporal temperature gradient gel electrophoresis; chicken microbiota; probiotics; lactobacillus;
D O I
10.1159/000106090
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A probiotic Lactobacillus strain was given in drinking water to young broiler chickens from 1 to 19 days of age. Cecal contents were collected from 4- and 19-day-old chickens in treated and control groups. Enumeration of bacteria by culture on selective media showed a decrease in Clostridium perfringens carriage in the 4-day-old treated chickens, whereas coliforms and Lactobacillus populations were not significantly affected by the treatment. Fluorescent in situ hybridization analysis with 7 phylogenetic probes targeting the major groups of intestinal bacteria revealed that the Clostridium coccoides group accounted for more than 50% of the total bacteria in the cecum of 4-day-old chickens, whereas the bacterial community of 19-day-old chickens evolved towards a more diverse microbiota with Faecalibacterium prausnitzii (36%) and C. coccoides (22%) groups representing the predominant bacteria. No effect of the Lactobacillus strain supplementation was observed in the composition of the cecal microbiota assessed by fluorescent in situ hybridization with the 7 probes. Nevertheless, profiling of the cecal microbiota using temporal temperature gradient gel electrophoresis in combination with principal component analysis demonstrated an impact of the probiotic treatment on the overall bacterial community as well as on the Lactobacillus population. Copyright (c) 2008 S. Karger AG, Basel.
引用
收藏
页码:115 / 122
页数:8
相关论文
共 41 条
[11]   Molecular microbial diversity of an anaerobic digestor as determined by small-subunit rDNA sequence analysis [J].
Godon, JJ ;
Zumstein, E ;
Dabert, P ;
Habouzit, F ;
Moletta, R .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (07) :2802-2813
[12]  
Gong JH, 2002, FEMS MICROBIOL LETT, V208, P1, DOI 10.1111/j.1574-6968.2002.tb11051.x
[13]  
Harmsen Hermie J.M., 1999, Microbial Ecology in Health and Disease, V11, P3, DOI 10.1080/089106099435862
[14]   Development of 16S rRNA-based probes for the Coriobacterium group and the Atopobium cluster and their application for enumeration of Coriobacteriaceae in human feces from volunteers of different age groups [J].
Harmsen, HJM ;
Wildeboer-Veloo, ACM ;
Grijpstra, J ;
Knol, J ;
Degener, JE ;
Welling, GW .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (10) :4523-4527
[15]   Effects of adherent Lactobacillus cultures on growth, weight of organs and intestinal microflora and volatile fatty acids in broilers [J].
Jin, LZ ;
Ho, YW ;
Abdullah, N ;
Ali, MA ;
Jalaludin, S .
ANIMAL FEED SCIENCE AND TECHNOLOGY, 1998, 70 (03) :197-209
[16]  
Kaldhusdal M., 2000, WORLD POULT, V16, P50
[17]   Effects of dietary fat source and subtherapeutic levels of antibiotic on the bacterial community in the ileum of broiler chickens at various ages [J].
Knarreborg, A ;
Simon, MA ;
Engberg, RM ;
Jensen, BB ;
Tannock, GW .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (12) :5918-5924
[18]   In vivo characterization of Lactobacillus johnsonii FI9785 for use as a defined competitive exclusion agent against bacterial pathogens in poultry [J].
La Ragione, RM ;
Narbad, A ;
Gasson, MJ ;
Woodward, MJ .
LETTERS IN APPLIED MICROBIOLOGY, 2004, 38 (03) :197-205
[19]   QUANTITATIVE FLUORESCENCE IN-SITU HYBRIDIZATION OF BIFIDOBACTERIUM SPP WITH GENUS-SPECIFIC 16S RIBOSOMAL-RNA-TARGETED PROBES AND ITS APPLICATION IN FECAL SAMPLES [J].
LANGENDIJK, PS ;
SCHUT, F ;
JANSEN, GJ ;
RAANGS, GC ;
KAMPHUIS, GR ;
WILKINSON, MHF ;
WELLING, GW .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (08) :3069-3075
[20]   Diversity and succession of the intestinal bacterial community of the maturing broiler chicken [J].
Lu, JR ;
Idris, U ;
Harmon, B ;
Hofacre, C ;
Maurer, JJ ;
Lee, MD .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (11) :6816-6824