Efficient osteoblastic differentiation of human adipose-derived stem cells on TiO2 nanoparticles and metformin co-embedded electrospun composite nanofibers

被引：25

作者：

Ahmadi, Somayeh ^{[1
]}

Pilehvar, Younes ^{[2
]}

Zarghami, Nosratollah ^{[3
]}

Abri, Abdolreza ^{[1
]}

机构：

[1] Azarbaijan Shahid Madani Univ, Dept Chem, Fac Sci, Tabriz 5165665811, Iran

[2] Urmia Univ Med Sci, Cellular & Mol Med Inst, Cellular & Mol Res Ctr, Orumiyeh, Iran

[3] Tabriz Univ Med Sci, Dept Clin Biochem & Lab Med, Fac Med, Tabriz, Iran

来源：

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY | 2021年 / 66卷

关键词：

Human adipose-derived stem cells; Osteoblastic differentiation; Electrospun nanofibers; Metformin; TUNABLE PHYSICOCHEMICAL PROPERTIES; NANO-ENCAPSULATED METFORMIN; EFFECTIVE GROWTH-INHIBITION; HYDROGEL SCAFFOLDS; HTERT EXPRESSION; PROLIFERATION; PRESERVATION;

D O I：

10.1016/j.jddst.2021.102798

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In the present study, TiO2 nanoparticle (nTiO2) and metformin (MET)-co-embedded composite electrospun poly epsilon-Caprolactone/gelatin nanofibers (PCL/GEL NFs) were developed as functional biomimetic scaffolds for improved osteoblastic differentiation of human adipose-derived stem cells (hADSCs). Morphological features and chemical configuration of fabricated electrospun fibers were characterized via FE-SEM, TEM, TGA, and FTIR. Improved mechanical properties were detected on the composite NFs due to the presence of inorganic particles, nTiO2. Besides, FE-SEM images and PicoGreen assay were demonstrated the enhanced adhesion and proliferation rate of hADSCs grown on the nTiO2/MET-loaded NFs, respectively. Moreover, a synergistic effects of nTiO2generated surface nanotopography and the released MET led to a great capacity in the scaffold to induce differentiation toward osteoblasts via enhanced mineralization content and increased expression levels of osteoblastic markers. Overall, this preliminary work demonstrated the nanocomposite scaffold can be deemed as a strong potential candidate for bone tissue regeneration and engineering.

引用

页数：11