Knockdown of p-Coumaroyl Shikimate/Quinate 3′-Hydroxylase Delays the Occurrence of Post-Harvest Physiological Deterioration in Cassava Storage Roots

被引：12

作者：

Ma, Qiuxiang ^{[1
]}

Xu, Jia ^{[1
]}

Feng, Yancai ^{[1
,2
]}

Wu, Xiaoyun ^{[1
]}

Lu, Xinlu ^{[1
]}

Zhang, Peng ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Ctr Excellence Mol Plant Sci, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 16期

基金：

中国国家自然科学基金; 上海市自然科学基金;

关键词：

cassava; PPD; C3 ' H; scopoletin; scopolin; MANIHOT-ESCULENTA CRANTZ; OXIDATIVE STRESS; FUNCTIONAL-CHARACTERIZATION; BIOCHEMICAL-CHANGES; FERULOYL COA; SCOPOLETIN; HYDROXYLATION; ACCUMULATION; HYDROXYCOUMARINS; 3-HYDROXYLASE;

D O I：

10.3390/ijms23169231

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cassava storage roots are an important source of food, feed, and material for starch-based industries in many countries. After harvest, rapid post-harvest physiological deterioration (PPD) reduces their palatability and marketability. During the PPD process, vascular streaking occurs through over-accumulation of coumarins, the biosynthesis of which involves the key enzyme p-coumaroyl shikimate/quinate 3'-hydroxylase (C3'H). Repression of MeC3'H expression by RNA interference in transgenic cassava plants caused a significant delay in PPD by decreasing scopoletin and scopolin accumulation in field-harvested storage roots. This study demonstrates that MeC3'H is the key enzyme participating in coumarin biosynthesis during PPD and shows that MeC3'H is a useful target gene for editing to prolong the shelf life of cassava storage roots.

引用

页数：12

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