More keys to padlock probes:: mechanisms for high-throughput nucleic acid analysis

被引：28

作者：

Banér, J ^{[1
]}

Nilsson, M ^{[1
]}

Isaksson, A ^{[1
]}

Mendel-Hartvig, M ^{[1
]}

Antson, DO ^{[1
]}

Landegren, U ^{[1
]}

机构：

[1] Rudbeck Lab, Dept Genet & Pathol, Beijer Lab, SE-75185 Uppsala, Sweden

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2001年 / 12卷 / 01期

关键词：

D O I：

10.1016/S0958-1669(00)00174-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

With the impending availability of total information about nucleic acid sequences in humans and other organisms, tools to investigate these sequences on a large scale assume increasing importance. Methods currently in use, however, cannot offer the required combination of high-throughput, sensitivity and specificity of detection. Padlock probes, circularizing oligonucleotides, may provide a means to detect, distinguish, quantitate and also locate very large numbers of DNA or RNA sequences. Recent developments in areas such as the biochemistry of ligation and characterization of ligases, methods to replicate circularized probes and the development of assays based on these principles augment the potential of padlock probes.

引用

页码：11 / 15

页数：5

共 44 条

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