Genome science-based gene expression monitoring in osteoblasts altered by low-level laser irradiation

Although the biostimulatory effects of low-level laser irradiation (LLLI) have been reported, the molecular basis of the mechanisms are not fully elucidated. To elucidate the mechanism of bone regeneration by low-level laser irradiation (LLLI), gene expressions altered in osteoblast by LLLI were identified using the subtractive gene cloning and cDNA microarray technologies. Many gene expressions in osteoblast were altered by LLLI Many genes whose expression levels were altered by LLLI were shown, including ATP synthesis, DNA replication, signal transduction, enzymes, structural proteins, ESTs and other unknown function genes. These findings suggest that LLLI may demonstrate biostimulatory effects through the expression of many genes, and genome science-based gene expression monitoring will provide unprecedented access to elucidate the mechanism of bone regeneration stimulated by LLLI. (C) 2002 Elsevier Science B.V. All rights reserved.