A Genetically Encoded FRET Sensor for Intracellular Heme

被引：63
|
作者：
Song, Yanqun [1 ]
Yang, Maiyun [1 ]
Wegner, Seraphine V. [1 ,2 ,3 ]
Zhao, Jingyi [4 ]
Zhu, Rongfeng [5 ]
Wu, Yun [6 ]
He, Chuan [1 ,2 ,3 ,7 ]
Chen, Peng R. [1 ,5 ,7 ]
机构：
[1] Peking Univ, Beijing Natl Lab Mol Sci, Synthet & Funct Biomol Ctr, Key Lab Bioorgan Chem & Mol Engn,Minist Educ,Coll, Beijing 100871, Peoples R China
[2] Univ Chicago, Dept Chem, Chicago, IL 60637 USA
[3] Univ Chicago, Inst Biophys Dynam, Howard Hughes Med Inst, Chicago, IL 60637 USA
[4] Peking Univ, Sch Chem Biol & Biotechnol, Lab Chem Genom, Shenzhen Grad Sch, Shenzhen 518055, Peoples R China
[5] Peking Tinghua Ctr Life Sci, Beijing, Peoples R China
[6] Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
[7] Shanghai Univ E Inst Chem Biol, Shanghai, Peoples R China
来源：
ACS CHEMICAL BIOLOGY | 2015年 / 10卷 / 07期
基金：
中国国家自然科学基金; 美国国家科学基金会;
关键词：
FLUORESCENT PROTEIN; OXYGENASE;
D O I：
10.1021/cb5009734
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Heme plays pivotal roles in various cellular processes as well as in iron homeostasis in living systems. Here, we report a genetically encoded fluorescence resonance energy transfer (FRET) sensor for selective heme imaging by employing a pair of bacterial heme transfer chaperones as the sensory components. This heme-specific probe allows spatial-temporal visualization of intracellular heme distribution within living cells.
引用
收藏
页码：1610 / 1615
页数：6
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