ASSOCIATION BETWEEN IL-4 GENE POLYMORPHISMS AND CHRONIC OBSTRUCTIVE PULMONARY DISEASE OF PATIENTS IN HEBEI PROVINCE, CHINA

We aimed to study the association between IL-4 gene polymorphisms and chronic obstructive pulmonary disease (COPD) of patients in Hebei Province. The blood samples of 62 COPD patients and 301 healthy subjects were collected. IL-4 gene polymorphisms were detected by PCR with sequence-specific primer typing. There were significant differences in the alleles of IL-4-33, IL-4-590 and IL-4-1098 between COPD patients and healthy subjects (P<0.05). IL-4-590/C (P<0.001, OR = 4.619, CI = 1.640-7.985) had the highest susceptibility, suggesting that people with this allele were 3.6 times more prone to COPD than those with IL-4-33-590/T allele. COPD of susceptible patients was positively associated with IL-4-33/C:C (P<0.001, OR = 5.3, CI = 0.939-7.714), IL-4-590/C:C (P<0.001, OR = 29.5, CI = 11.138-57.647) and IL-4-1098/T:T (P<0.001, OR = 3.3, CI = 1.016-7.862). According to the level of protectiveness, COPD of patients (protective) was negatively associated with IL-4-33/C:T (P = 0.009, OR = 0.109, CI = 0.015-0.809), IL-4-590/C:T (P = 0.009, OR = 0.397, CI = 0.195-0.810), IL-4-1098/G:G (P<0.001, OR = 0.133, CI = 0.054-0.331) and IL-4-1098/G:T (P<0.001, OR = 0.209, CI = 0.104-0.527). IL-4/TCT (P<0.001, OR = 35.500, CI = 11.30-111.515) was found in susceptible patients. Negative association was found between COPD and haplotypes: IL-4/TTC (P<0.001, OR = 0.061, CI=0.008-0.443) and IL-4/GCC (P<0.001, OR = 0.193, CI = 0.076-0.488). IL-4/GCT, IL-4/GTC and IL-4/GTT were only found in healthy subjects. IL-4 gene polymorphisms (loci 1098, -590 and -33) were closely associated with COPD in Hebei Province.