Long non-coding RNA HNF1A-AS1 promotes cell proliferation and invasion via regulating miR-17-5p in non-small cell lung cancer

被引：57

作者：

Zhang, Guoyu ^{[1
,2
]}

An, Xiaokang ^{[2
]}

Zhao, Huiya ^{[3
]}

Zhang, Qingyong ^{[1
]}

Zhao, Hui ^{[4
]}

机构：

[1] Zhengzhou Univ, Affiliated Hosp 2, Dept Thorac Surg, 2 Jingba Rd, Zhengzhou 450014, Henan, Peoples R China

[2] Henan Univ, Affiliated Hosp 1, Dept Thorac Surg, Kaifeng 475000, Henan, Peoples R China

[3] Childrens Hosp Kaifeng City, Dept Infect, Kaifeng 475000, Henan, Peoples R China

[4] Henan Univ, Huaihe Hosp, Dept Cardiothorac Surg, Kaifeng 475000, Henan, Peoples R China

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2018年 / 98卷

关键词：

LncRNA; HNF1A-AS1; Lung cancer; miR-17-5p; HEPATOCELLULAR-CARCINOMA; DOWN-REGULATION; UP-REGULATION; METASTASIS; EPIDEMIOLOGY; EXPRESSION;

D O I：

10.1016/j.biopha.2017.12.080

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Long non-coding RNA HNF1A-antisense 1 (lncRNA HNF1A-AS1) plays important roles in the progression of human tumors. The aim of this study is to unravel the underlying mechanism of HNF1A-AS1 in non-small cell lung cancer (NSCLC). In the present study, we found that HNF1A-AS1 was upregulation in NSCLC tissues and cell lines. High HNF1A-AS1 expression was associated with patients' advanced TNM stage and lymph node metastasis. Reduced HNF1A-AS1 expression inhibited lung cancer cells proliferation, invasion and increased cells apoptosis rate. Bioinformatics analysis and luciferase reporter assay revealed that HNF1A-AS1 interacted with miR-17-5p by directly targeting it. Rescue experiments showed that miR-17-5p suppression reversed the tumor-suppressing role of HNF1A-AS1 knockdown on NSCLC progression. Conclusion, our data indicated that lncRNA HNF1A-AS1 promoted lung cancer cells proliferation and invasion via regulating miR-17-5p, suggesting that HNF1A-AS1 could act as a potent therapeutic strategy for the treatment of NSCLC patients.

引用

页码：594 / 599

页数：6

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