Nucleobase recognition at alkaline pH and apparent pKa of single DNA bases immobilised within a biological nanopore

被引：23

作者：

Franceschini, Lorenzo ^{[1
]}

Mikhailova, Ellina ^{[2
]}

Bayley, Hagan ^{[2
]}

Maglia, Giovanni ^{[1
]}

机构：

[1] Univ Louvain, Dept Chem, B-3001 Louvain, Belgium

[2] Univ Oxford, Dept Chem, Oxford OX1 3TA, England

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 10期

基金：

欧洲研究理事会;

关键词：

ION-CHANNEL; DISCRIMINATION; DENATURATION; SALT; RNA; TRANSLOCATION; PROTONATION; MOLECULES; VALUES; CHARGE;

D O I：

10.1039/c1cc16124e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The four DNA bases are recognized in immobilized DNA strands at high alkaline pH by nanopore current recordings. Ionic currents through the biological nanopores are also employed to measure the apparent pK(a) values of single nucleobases within the immobilised DNA strands.

引用

页码：1520 / 1522

页数：3

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