Chemiluminescent Determination of MicroRNA-141 Using Target-Dependent Activation of the Peroxidase-Mimicking DNAzyme

被引：4
作者：
Bodulev, Oleg L. [1 ]
Sakharov, Ivan Yu [1 ]
机构：
[1] Lomonosov Moscow State Univ, Dept Chem, Moscow 119991, Russia
来源：
ANALYTICAL LETTERS | 2019年 / 52卷 / 05期
基金：
俄罗斯科学基金会;
关键词：
Chemiluminescence; microRNA-141; peroxidase mimetics; peroxidase-mimicking DNAzyme; SIGNAL AMPLIFICATION; GOLD NANOPARTICLES; GRAPHENE OXIDE; DNA; CANCER; FLUORESCENCE; BIOMARKERS; BIOSENSORS; REGULATOR; DIAGNOSIS;
D O I：
10.1080/00032719.2018.1498506
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A chemiluminescent method was developed for microRNA-141 (miRNA-141) detection based on the target-dependent activation of peroxidase-mimicking DNAzyme. The structure of the probes was optimized which allowed the development of a sensitive method for miRNA-141. Under the optimized conditions, the detection limit and the linear range were 100 pM and 0.1-50 nM, respectively. The sensitivity of the assay was 270,000 nM(-1). The values of coefficient of variation measured within the working range varied less than 2%, which indicates excellent precision for the proposed method.
引用
收藏
页码：813 / 824
页数：12
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