Competitive binding of Cd and Zn with the phytochelatin (γ-Glu-Cys)4-Gly:: Comparative study by mass spectrometry, voltammetry-multivariate curve resolution, and isothermal titration calorimetry

被引：38

作者：

Chekmeneva, Elena ^{[1
]}

Prohens, Rafel ^{[2
]}

Diaz-Cruz, Jose Manuel ^{[1
]}

Arino, Cristina ^{[1
]}

Esteban, Miquel ^{[1
]}

机构：

[1] Univ Barcelona, Fac Quim, Dept Quim Analit, E-08028 Barcelona, Spain

[2] Univ Barcelona, Unidad Quim Fina, Serv Cientif Tecn, E-08028 Barcelona, Spain

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 2008年 / 42卷 / 08期

关键词：

D O I：

10.1021/es702725a

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The competitive binding of Cd2+ and Zn2+ by the phytochelatin (gamma-Glu-Cys)(4)-Gly (PC4) has been examined using several techniques. Electrospray ionization mass spectrometry is used to determine the stoichiometries of the complexes, while voltammetric data analyzed by multivariate curve resolution with alternating least squares allows one to not only trace the displacement induced by Cd2+ or Zn2+ in the binding of Zn2+ or Cd2+, respectively, by PC4, but also to obtain a complete overview of the processes involved. Isothermal titration calorimetry is used to determine the related binding and thermodynamic parameters. Results obtained via these different techniques are compared and discussed below. The formation of ternary CdZn(PC4) and Cd2Zn(PC4) complexes was observed.

引用

页码：2860 / 2866

页数：7

共 32 条

[1] Trace metal control of phytochelatin production in coastal waters [J].

Ahner, BA ;

Morel, FMM ;

Moffett, JW .

LIMNOLOGY AND OCEANOGRAPHY, 1997, 42 (03) :601-608

[2] PHYTOCHELATIN PRODUCTION IN MARINE-ALGAE .1. AN INTERSPECIES COMPARISON [J].

AHNER, BA ;

KONG, S ;

MOREL, FMM .

LIMNOLOGY AND OCEANOGRAPHY, 1995, 40 (04) :649-657

[3] PHYTOCHELATIN PRODUCTION IN MARINE-ALGAE .2. INDUCTION BY VARIOUS METALS [J].

AHNER, BA ;

MOREL, FMM .

LIMNOLOGY AND OCEANOGRAPHY, 1995, 40 (04) :658-665

[4] Metal ion ligands in hyperaccumulating plants [J].

Callahan, DL ;

Baker, AJM ;

Kolev, SD ;

Wedd, AG .

JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2006, 11 (01) :2-12

[5]

CHEKMENEVA E, ANAL BIOCH

[6] Binding of Cd2+ and Zn2+ with the phytochelatin (γ-Glu-Cys)4-Gly:: a voltammetric study assisted by multivariate curve resolution and electrospray ionization mass spectrometry [J].

Chekmeneva, Elena ;

Diaz-Cruz, Jose Manuel ;

Arino, Cristina ;

Esteban, Miquel .

ELECTROANALYSIS, 2007, 19 (2-3) :310-317

[7] Phytochelatins and their roles in heavy metal detoxification [J].

Cobbett, CS .

PLANT PHYSIOLOGY, 2000, 123 (03) :825-832

[8] Complexation of heavy metals by phytochelatins:: Voltammetric study of the binding of Cd2+ and Zn2+ ions by the phytochelatin (γ-Glu-Cys)3Gly assisted by multivariate curve resolution [J].

Cruz, BH ;

Díaz-Cruz, JM ;

Ariño, C ;

Esteban, M .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2005, 39 (03) :778-786

[9] Study of complexing properties of the α and β metallothionein domains with cadmium and/or zinc using electrospray ionisation mass spectrometry [J].

Dabrio, M ;

Van Vyncht, G ;

Bordin, G ;

Rodriguez, AR .

ANALYTICA CHIMICA ACTA, 2001, 435 (02) :319-330

[10] Cadmium uptake and translocation in tumbleweed (Salsola kali), a potential Cd-hyperaccumulator desert plant species:: ICP/OES and XAS studies [J].

de la Rosa, G ;

Peralta-Videa, JR ;

Montes, M ;

Parsons, JG ;

Cano-Aguilera, I ;

Gardea-Torresdey, JL .

CHEMOSPHERE, 2004, 55 (09) :1159-1168

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