Second-harmonic imaging of plant polysaccharides -: art. no. 024013

被引：77
作者：
Cox, G
Moreno, N
Feijó, J
机构：
[1] Univ Sydney, Electron Microscope Unit, Sydney, NSW 2006, Australia
[2] Inst Gulbenkian Ciencias, PT-2780156 Oeiras, Portugal
来源：
JOURNAL OF BIOMEDICAL OPTICS | 2005年 / 10卷 / 02期
关键词：
second-harmonic generation; polysaccharides; cellulose; starch three-photon fluorescence; food microscopy;
D O I：
10.1117/1.1896005
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The application of second-harmonic generation (SHG) microscopy to plant materials has been neglected hitherto even though it would seem to have promise for identification and characterization of biologically and commercially important plant polysaccharides. We find that imaging of cellulose requires rather high laser powers, which are above optimal values for live cell imaging. Starch, however, is easily imaged by the technique at laser fluences compatible with extended cell viability. This also has useful applications in imaging plant-derived starchy food products. Lignin in plant cell walls shows a strong three-photon excited fluorescence, which may be enhanced by resonance effects. (c) 2005 Society of Photo-Optical Instrumentation Engineers.
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页数：6
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