Expression of Lactococcus lactis NADH oxidase increases 2,3-butanediol production in Pdc-deficient Saccharomyces cerevisiae

被引：48

作者：

Kim, Jin-Woo ^{[1
,2
]}

Seo, Seung-Oh ^{[3
,4
]}

Zhang, Guo-Chang ^{[3
,4
]}

Jin, Yong-Su ^{[3
,4
]}

Seo, Jin-Ho ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, Dept Agr Biotechnol, Seoul 151921, South Korea

[2] Seoul Natl Univ, Ctr Food & Bioconvergence, Seoul 151921, South Korea

[3] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA

[4] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA

来源：

BIORESOURCE TECHNOLOGY | 2015年 / 191卷

基金：

新加坡国家研究基金会;

关键词：

Saccharomyces cerevisiae; 2,3-Butanediol; Glycerol; NADH oxidase; Cofactor engineering; PYRUVATE-DECARBOXYLASE; BACILLUS-SUBTILIS; GLUCOSE; YEAST; DEHYDROGENASE; METABOLISM; ACETOIN; IMPACT; FERMENTATION; KINASE;

D O I：

10.1016/j.biortech.2015.02.077

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

To minimize glycerol production during 2,3-BD fermentation by the engineered Saccharomyces cerevisiae, the Lactococcus lactis water-forming NADH oxidase gene (noxE) was expressed at five different levels. The expression of NADH oxidase substantially decreased the intracellular NADH/NAD(+) ratio. The S. cerevisiae BD5_T2nox strain expressing noxE produced 2,3-BD with yield of 0.359 g 2,3-BD/g glucose and glycerol with 0.069 g glycerol/g glucose, which are 23.8% higher and 65.3% lower than those of the isogenic strain without noxE. These results demonstrate that the carbon flux could be redirected from glycerol to 2,3-BD through alteration of the NADH/NAD+ ratio by the expression of NADH oxidase. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：512 / 519

页数：8

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