Registration of extra-hard kernel near-isogenic hexaploid wheat genetic stocks lacking puroindoline genes

Kernel hardness (texture) is a key end-use quality trait of wheat (Triticum spp.). Two hexaploid wheat (T. aestivum L.) near-isogenic lines (NILs), WQL18RE5 (Reg. no. GS-190, PI 691609) and WQL18CS5 (Reg. no. GS-191, PI 691610), lack the puroindoline genes at the Hardness locus and thus have kernel texture phenotypes more similar to durum wheat [T. turgidum subsp. durum (Desf.) Husn.]. These two genetic stocks were produced in `Alpowa' soft white spring wheat and comprise additions to an ongoing series of NILs in this soft wheat cultivar. Both NILs are advanced generation BC7F2:5 derivatives (Alpowa/donor/7*Alpowa). WQL18RE5 was derived from a Chinese Spring (CS) disomic substitution line, CS (Red Egyptian 5D), and WQL18CS5 was derived from a CS deletion line (CS5DS del.-2) that lacks the distal portion of the short arm of chromosome 5D. These lines were developed at the USDAARS-Western Wheat Quality Laboratory and will be useful to study kernel texture and end-use quality in hexaploid wheat; they can also serve as parents in developing cultivars with kernel texture equivalent to that of durum wheat.