Glioblastoma Factors Increase the Migration of Human Brain Endothelial Cells In Vitro by Increasing MMP-9/CXCR4 Levels

被引：13

作者：

De Oliveira Rosario, Luciane Vieira ^{[1
]}

Da Rosa, Barbara Gomes ^{[2
]}

Goncalves, Thaynan Lopes ^{[1
]}

Lourenco Matias, Diana Isabel ^{[3
]}

Freitas, Catarina ^{[2
]}

Ferrer, Valeria Pereira ^{[4
]}

机构：

[1] Paulo Niemeyer State Brain Inst, Brains Biomed Lab, Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Biomed Sci Inst, Cellular Morphogenesis Lab, Rio De Janeiro, Brazil

[3] UCL, Dept Chem, Battaglias Lab, Christopher Ingold Bldg, London, England

[4] Fluminense Fed Univ, Inst Biol, Dept Cellular & Mol Biol, 30 Mario Santos Braga St, Niteroi, RJ, Brazil

来源：

ANTICANCER RESEARCH | 2020年 / 40卷 / 05期

关键词：

Angiogenesis; endothelial cells; glioblastoma; cell crosstalk; MMP-9; CXCR4; GROWTH-FACTOR RECEPTOR-2; TUMOR ANGIOGENESIS; MESSENGER-RNA; UP-REGULATION; TENASCIN-C; CROSS-TALK; VEGF; EXPRESSION; PROLIFERATION; INVASION;

D O I：

10.21873/anticanres.14244

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background/Aim: Glioblastoma (GB) is the most aggressive type of tumor in the central nervous system and is characterized by resistance to therapy and abundant vasculature. Tumor vessels contribute to the growth of GB, and the tumor microenvironment is thought to influence tumor vessels. We evaluated the molecular communication between human GB cells and human brain microvascular endothelial cells (HBMEC) in vitro. Materials and Methods: We investigated whether GB-conditioned media (GB-CM) influenced HBMEC proliferation and migration, as well as the levels of MMP-9, CXCL12, CXCR4, CXCR7, VEGFs, VEGFR-2, and WNT5a in HBMEC. Results: Although HBMEC proliferation was not modified, increased HBMEC migration was detected after GB-CM treatment. Furthermore, treatment of HBMEC with GB-CM resulted in increased levels of MMP-9 and CXCR4. The levels of WNT5a, VEGFs and VEGFR-2 were not affected. Conclusion: GB-secreted factors lead to increased endothelial cell migration and to increased levels of MMP-9 and CXCR4.

引用

页码：2725 / 2737

页数：13

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